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目的调查临床内镜复用过程中细菌生物膜的污染情况,检测生物膜清洗液在内镜清洗消毒中对细菌生物膜的清洗效果。方法参照GB15982和WS507《软式内镜清洗消毒技术规范》监测2017年1月-2017年3月临床80例内镜使用后的消毒效果,并采用活菌计数法评价内镜生物膜去除效果。结果在高水平消毒合格的前提下,10.0%电子胃镜检出细菌生物膜,15.0%电子肠镜检出细菌生物膜。生物膜清洗液对电子胃镜和电子肠镜细菌生物膜的去除率高达99.0%以上,能彻底清除内镜腔体内形成的细菌生物膜,保证后续消毒(灭菌)效果。结论与常规多酶清洗液相比,生物膜清洗液能有效去除内镜腔内的细菌生物膜,保证了消毒(灭菌)效果,避免医院感染。
Objective To investigate the contamination of bacterial biofilm during clinical endoscopic re-use and to examine the biofilm cleaning effect of biofilm cleaning solution during endoscopic cleaning and disinfection. Methods According to GB15982 and WS507 “soft endoscope cleaning and disinfection technical specifications” to monitor the clinical 2017 January 2017 -2017 clinical use of 80 cases of endoscopic disinfection, and the use of viable count evaluation of endoscopic biofilm removal. Results In the high level of disinfection under the premise of 10.0% electron gastroscopy bacterial biofilm detection, 15.0% colonoscopy biofilm detection. Biofilm cleaning fluid on the electronic endoscopy and colonoscopy of bacterial colon biofilm removal rate as high as 99.0%, to completely clear the endoscopic cavity formed in the bacterial biofilm, to ensure follow-up disinfection (sterilization) effect. Conclusion Compared with the conventional multi-enzyme washing solution, the biofilm washing solution can effectively remove the bacterial biofilm in the endoscopic cavity to ensure the disinfection (sterilization) effect and avoid nosocomial infection.