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目的:证明胃癌血管靶向肽GX2能够与胃癌血管内皮细胞特异性结合,在体内对胃癌血管具有靶向性。方法:体外实验,合成GX2与FITC的复合物FITC-GX2,分离原代人脐静脉内皮细胞HUVEC,将HUVEC与人胃癌细胞系SGC7901共培养,建立共培养血管内皮细胞模型来模拟胃癌血管,利用免疫荧光的方法观察GX2与共培养内皮细胞Co-HUVEC的结合情况;体内实验,构建99mTc标记的GX2分子示踪探针,SPECT显像观察GX2的胃癌血管靶向性。结果:免疫荧光结果显示GX2能与Co-HUVEC特异性结合,而与人胃癌细胞SGC7901不结合;SPECT显像结果证明了GX2在荷瘤裸鼠体内能够浓集到肿瘤部位,具有良好的靶向性。结论:GX2能与胃癌血管内皮细胞特异性结合,且具有在体靶向到胃癌血管的能力;GX2具有胃癌诊断的潜在价值,并能应用于胃癌血管抑制治疗。
Objective: To prove that gastric cancer vascular targeting peptide GX2 can specifically bind to gastric cancer vascular endothelial cells, and has targeted on gastric cancer in vivo. METHODS: FITC-GX2, a complex of GX2 and FITC, was synthesized in vitro. Primary human umbilical vein endothelial cells (HUVECs) were isolated and cultured. Human HUVECs were co-cultured with human gastric cancer cell line SGC7901 to establish co-cultured vascular endothelial cells Immunofluorescence method was used to observe the binding of GX2 to co-cultured endothelial cells Co-HUVEC. In vivo experiments, 99mTc-labeled GX2 molecular probe and SPECT imaging were used to observe the vascular targeting of GX2. Results: The results of immunofluorescence showed that GX2 could specifically bind to Co-HUVEC but not to human gastric cancer cell line SGC7901. The results of SPECT showed that GX2 could reach the tumor site in tumor-bearing nude mice with good targeting Sex. CONCLUSION: GX2 can specifically bind to vascular endothelial cells of gastric cancer and has the potential of targeting in vivo to the vessels of gastric cancer. GX2 has the potential value of gastric cancer diagnosis and can be used in vascular suppression of gastric cancer.