肝功衰竭时有哪些代谢变化与脑病的发生有关,一直是争论热烈的课题。在Reese和Karnovsky经典的超微结构研究以前,血脑屏障(BBB)的解剖部位始终未明,甚至屏障的存在也有人怀疑,对于脑组织中是否存在间质间隙这一问题也认识混乱。Reese等应用辣根过氧化物酶(HRP)作为电子致密的反应产物,发现蛋白质示踪物不能穿透脑毛细血管内皮细胞的紧密连接。静脉注射时,HRP限制在血管腔内,不能入脑;相反,若置于脑脊液时,则HRP经由星形细胞终足 What metabolic changes during liver failure are associated with the occurrence of encephalopathy has been a hot topic of debate. Before Reese and Karnovsky’s classic ultrastructural studies, the anatomy of the blood-brain barrier (BBB) ​​remained unknown. Even the presence of a barrier was suspect, as was the confusion about the presence of interstitial space in brain tissue. Reese et al. Used horseradish peroxidase (HRP) as an electron-dense reaction product and found that protein tracers failed to penetrate the tight junctions of brain capillary endothelial cells. When injected intravenously, HRP is confined within the lumen of the blood vessel and not into the brain; conversely, when placed in cerebrospinal fluid, HRP is passed via astrocytes