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早年知道,1,25-二羟基维生素D_3〔1,25(OH)_2D_3〕参与钙磷骨骼代谢;近年又发现,1,25(OH)_2D_3具有更广泛的生物学作用.正常人单核细胞及恶性淋巴细胞均有1,25(OH)_2D_3受体;然而休止T、B细胞并无此受体,但被致分裂原活化或EB病毒感染后即可表达.本文报道,1,25(OH)_2D_3抑制PHA诱导的淋巴细胞增殖与IL_2生成.正常成人PBL、1%PHA及浓度递增的组胺(16~(-13)～10~(-8)M)在37℃共孵48小时,上清液中IL_2含量采用Smith等的生物测定法测定;淋巴细胞增殖以~3H-TdR
In the early years, 1,25-dihydroxyvitamin D 3 〔1,25 (OH) _2D_3〕 involved in calcium and phosphorus bone metabolism; recently found that 1,25 (OH) _2D_3 has a broader biological role in normal human monocytes And malignant lymphocytes have 1,25 (OH) _2D_3 receptor; However, there is no such receptor in T and B cells, but it can be expressed by mitogen-activated or Epstein-Barr virus infection.In this paper, OH) _2D_3 inhibits PHA-induced lymphocyte proliferation and IL-2 production.Methods: Normal adult PBL, 1% PHA and increasing concentrations of histamine (16 ~ (13) -10 ~ (-8) M) , IL-2 content in supernatant was determined by bioassay of Smith et al. Lymphocyte proliferation was measured with ~ 3H-TdR