论文部分内容阅读
采用硅胶柱色谱、Sephadex LH-20、HPLC等柱色谱技术,从南方红豆杉细胞培养物的80%乙醇(体积分数)提取物中分离得到13个化合物,根据理化性质与波谱数据鉴定化合物的结构分别为:2α,4α,7β,9α,10β-pentaacetoxy-14β-hydroxytax-11-ene(1)、2α,4α,7β,9α,10β-pentaacetoxytax-11-ene(2)、1β-deoxybaccatin VI(3)、2α-acetoxytaxusin(4)、taxuyunnanine C(5)、yunnanxane(6)、2α,5α,10β-triacetoxy-14β-propionyloxy-4(20),11-taxadiene(7)、2α,5α,10β-triacetoxy-14β-isobutyryloxy-4(20),11-taxadiene(8)、2α,5α,10β-triacetoxy-14β-(2’-methyl)butyryloxy-4(20),11-taxadiene(9)、13-dehydroxylbaccatin III(10)、13-dehydroxy-10-deacetylbaccatin III(11)、paclitaxel(12)及β-谷甾醇(13)。其中,化合物1为新化合物,化合物2、4、10和11为首次从南方红豆杉的细胞培养物中分离得到。
Thirteen compounds were isolated from 80% ethanol (volume fraction) extracts of Taxus chinensis var. Mairei cell culture by column chromatography on silica gel, Sephadex LH-20 and HPLC. The structures of the compounds were identified by their physicochemical properties and spectral data (2), 1β-deoxybaccatin VI (2α, 4α, 7β, 9α, 10β-pentaacetoxytax-11-ene 3α, 2α-acetoxytaxusin (4), taxuyunnanine C (5), yunnanxane (6), 2α, 5α, 10β-triacetoxy-14β- propionyloxy-4 (20), 11-taxadiene -triacetoxy-14β-isobutyryloxy-4 (20), 11-taxadiene (8), 2α, 5α, 10β- triacetoxy-14β- -hydroxylbaccatin III (10), 13-dehydroxy-10-deacetylbaccatin III (11), paclitaxel (12) and β-sitosterol (13). Among them, compound 1 was a new compound, and compounds 2, 4, 10 and 11 were isolated from the cell culture of Taxus chinensis for the first time.