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目的探讨不同剂量弓形虫感染对BALB/c小鼠的生殖毒性作用。方法将50只受精鼠随机分为5组,每组10只;孕8 d,阴性对照组给予磷酸盐缓冲液,低、中、高剂量组腹腔注射50、100、200个/只纯化RH株弓形虫速殖子,阳性对照组给予环磷酰胺20 mg/kg;孕18 d观察孕鼠主要脏器及胚胎情况。结果阳性对照组吸收胎率(19.6%)、死胎率(23.6%)、流产率(4.6%)、畸胎率(31.4%)与阴性对照组比较明显升高,差异均有统计学意义(P<0.01)。弓形虫100、200个/只剂量组吸收胎率、死胎率、流产率分别为15.7%、8.2%、5.8%和28.9%、12.5%、15.1%,高于阴性对照组(P<0.05);200个/只剂量组活胎畸形率与阴性对照组比较(27.3%,0%)明显升高(P<0.01)。结论 100、200个/只纯化RH株刚地弓形虫速殖子孕中期感染可致BALB/c小鼠生殖毒性。
Objective To investigate the effects of different doses of Toxoplasma gondii on the reproductive toxicity of BALB / c mice. Methods Fifty fertilized mice were randomly divided into 5 groups with 10 rats in each group. Pregnant mice were dredged for 8 days. Phosphate buffer solution was given to the negative control group. 50, 100, 200 / Toxoplasma tachyzoites, positive control group given cyclophosphamide 20 mg / kg; pregnancy 18 d observed the main organs and embryos of pregnant rats. Results In the positive control group, the rate of abortion (19.6%), stillbirth (23.6%), miscarriage rate (4.6%) and teratogenic rate (31.4%) were significantly higher than those in the negative control group <0.01). Toxoplasma gondii at 100, 200 / dose groups were 15.7%, 8.2%, 5.8% and 28.9%, 12.5% and 15.1% respectively, which was higher than that of the negative control group (P <0.05). Compared with the negative control group, the rate of live birth deformity was significantly increased in 200 / dose groups (27.3%, 0%) (P <0.01). Conclusion The reproductive toxicity of BALB / c mice can be induced by the infection of tachyzoites of Toxoplasma gondii at 100,200 / purified RH strains during the second trimester of pregnancy.