论文部分内容阅读
Objective To investigate the role of matrix metalloproteinase-1(MMP-1)/proteaseactivated receptor-1(PAR-1) signaling in the cervical cancer invasion.Methods RT-PCR was used to test the mRNA level of MMP-1 in 59 cases of cervical squamous cell cancer. Cell invasion was evaluated by Transwell assay to explore the effect of adding human recombinant MMP-1(rhMMP-1) and PAR1-siRNA on cervical cancer invasion.Results In cervical cancer tissues, more MMP-1 expression was observed than that in the normal cervical tissues, and its expression correlated with cancer status. Human recombinant MMP-1(rhMMP-1) could promote Hela cell invasion, and its number of invasive cell correlated with the concentration of rhMMP-1. Disrupting the expression of PAR-1 reduced the MMP-1 promoting-effect on Hela cell invasion, but had no effect on non-MMP-1 invasive action.Conclusion The MMP-1/PAR-1 signaling pathway is involved in cervical cancer invasion. Therefore, blocking PAR-1 may represent a new therapeutic option for metastatic cervical cancer.
Objective To investigate the role of matrix metalloproteinase-1 (MMP-1) / proteaseactivated receptor-1 (PAR-1) signaling in the cervical cancer invasion. Methods RT-PCR was used to test the mRNA level of MMP-1 in 59 cases of cervical squamous cell cancer. Results in cervical cancer tissues, more MMP-1 expression was observed than that in the normal cervical tissues, and its expression correlated with cancer status. Human recombinant MMP-1 (rhMMP-1) could promote Hela cell invasion, and its number of invasive cells correlated with the concentration of rhMMP-1. expression of PAR-1 reduced the MMP-1 promoting-effect on Hela cell invasion, but had no effect on non-MMP-1 invasive action. Confc The MMP-1 / PAR-1 signaling pathway is involved in cervical cancer invasion. , blocking PAR-1 may represent a new therapeutic op tion for metastatic cervical cancer.