作者对90例患系统性红斑狼疮(SLE)、类风湿性关节炎(RA)、类风湿性关节炎的幼年型和其他伴有或不伴有免疫紊乱的结缔组织病患者的淋巴细胞作了核仁试验。方法是用pH5的缓冲甲苯胺蓝染色干燥未固定的血液涂片,数100个淋巴细胞,具有紧密的核仁和细网状结构的核仁的细胞作为活动性淋巴细胞(即具有合成核糖核酸的核仁的淋巴细胞)。从核仁试验所得的值及淋巴细胞总数计算每立方毫米血液中活动性淋巴细胞的绝对数。结果在 The authors made a lecture on lymphocytes from 90 patients with SLE, RA, rheumatoid arthritis and other connective tissue disease patients with or without immune disorders Nucleolus test. The method is to dry the unfixed blood smear with a buffer of toluidine blue (pH 5), and to count as many as 100 lymphocytes, cells with a tight nucleolus and a fine network of nucleolus as active lymphocytes (ie with synthetic ribonucleic acid Nucleolar lymphocytes). Calculate the absolute number of active lymphocytes per cubic millimeter of blood from the values ​​obtained from nucleolar tests and the total number of lymphocytes. The result is