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目的探讨重组腺病毒介导的FOXO/MDA-7对乳腺癌MDA-MB-231细胞凋亡的影响及其机制。方法将MDA-MB-231细胞分为Ad-FOXO/MDA-7组、Ad-FOXO组、Ad-MDA-7组和空白对照组,采用Real-time PCR、q RTPCR和Western blot法检测各组细胞中FOXO和MDA-7基因的转录及蛋白表达水平;MTT法检测细胞的增殖活力;流式细胞术检测细胞的凋亡情况;Transwell法检测细胞的侵袭能力;Western blot法检测细胞中Ras、Raf、MEK和ERK蛋白的表达情况。结果与其他各组相比,转染Ad-FOXO/MDA-7后,MDA-MB-231细胞中FOXO和MDA-7的表达明显增强,细胞的增殖活力和侵袭能力明显被抑制(P<0.05),凋亡率明显升高,细胞中Ras、Raf、MEK和ERK蛋白的表达也明显被抑制。结论重组腺病毒Ad-FOXO/MDA-7能明显促进乳腺癌细胞的凋亡,为乳腺癌的基因治疗提供了新的思路。
Objective To investigate the effect of recombinant adenovirus mediated FOXO / MDA-7 on the apoptosis of breast cancer MDA-MB-231 cells and its mechanism. Methods MDA-MB-231 cells were divided into Ad-FOXO / MDA-7 group, Ad-FOXO group, Ad-MDA-7 group and blank control group. Real-time PCR, qRTPCR and Western blot were used to detect the expression of MDA- The transcription and protein expression of FOXO and MDA-7 were detected by MTT assay. The apoptosis of cells was detected by flow cytometry. The invasion ability of cells was detected by Transwell assay. The expressions of Ras, Raf, MEK and ERK protein expression. Results Compared with other groups, the expression of FOXO and MDA-7 in MDA-MB-231 cells was significantly increased after transfection with Ad-FOXO / MDA-7 and the proliferation and invasion ability of cells were significantly inhibited (P <0.05 ), The apoptosis rate was significantly increased, and the expression of Ras, Raf, MEK and ERK in the cells was also significantly inhibited. Conclusion The recombinant adenovirus Ad-FOXO / MDA-7 can significantly promote the apoptosis of breast cancer cells and provide a new idea for the gene therapy of breast cancer.