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目的 研究海水浸泡对失血性休克大鼠血流动力学的影响 ,并探讨其改变机制。方法 采用雄性Wistar大鼠 ,分为 3组 :正常对照组 (n =8) ;平原休克组 (n =8) ;海水浸泡失血性休克组 (n =8)。测定血流动力学指标 ,血浆磷脂酶A2 (PLA2 )活性 ,心肌和心肌线粒体钙含量 ,心肌线粒体Ca2 + Mg2 + ATPase活性变化 ,血浆和心肌MDA的变化。结果 海水浸泡失血性休克动物血流动力学明显低于平原失血性休克组。海水浸泡失血性休克组动物心肌钙超载显著重于平原休克组 ,心肌和心肌线粒体钙含量均高于平原休克组 ,心肌线粒体Ca2 + Mg2 + ATPase活性显著低于平原休克组 ,心肌MDA显著高于平原休克组 ,血浆MDA和PLA2显著高于平原休克组。结论 低温海水浸泡失血性休克动物心肌及其线粒体钙明显超载、脂质过氧化及PLA2 升高可能是血流动力学下降的重要原因
Objective To study the effects of seawater immersion on hemodynamics in hemorrhagic shock rats and to explore its mechanism. Methods Male Wistar rats were divided into 3 groups: normal control group (n = 8), placental shock group (n = 8) and seawater immersion hemorrhagic shock group (n = 8). The hemodynamics, plasma phospholipase A2 (PLA2) activity, myocardial and myocardial mitochondrial calcium content, myocardial mitochondrial Ca2 + Mg2 + ATPase activity changes, plasma and myocardial MDA levels were measured. Results Hemodynamics in seawater immersion hemorrhagic shock animals was significantly lower than that in hemorrhagic shock group. Myocardial calcium overload in seawater immersion hemorrhagic shock group was significantly higher than that in plain shock group, and myocardial and myocardial mitochondrial calcium content was higher than that in plain shock group. Myocardial mitochondrial Ca2 + Mg2 + ATPase activity was significantly lower than plain shock group, and myocardial MDA was significantly higher Plain shock group, plasma MDA and PLA2 was significantly higher than the plain shock group. Conclusions Myocardial and mitochondrial calcium in hemorrhagic shock rats exposed to low temperature seawater are significantly overloaded. Lipid peroxidation and elevated PLA2 may be the important reasons for the decrease of hemodynamics