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目的采用HPLC法测定夏枯草中芦丁和槲皮素的含量。方法色谱柱为Alltima C18(250mm×4.6mm,5μm),流动相为甲醇-0.1%冰醋酸,检测波长350nm,流速1.0mL·min-1,柱温30℃。结果芦丁、槲皮素的线性范围分别为0.0149～0.2380mg·mL-1(r=0.9999)、0.0019～0.0306mg·mL-1(r=0.9998),平均回收率分别为97.78%(RSD=0.83%)、101.18%(RSD=0.83%)。安徽、湖北、贵州、江西、广西五产地中芦丁含量分别为0.3527、0、0.2337、2.1164、0.9578mg·g-1生药,槲皮素含量分别为0.0872、0.0473、0.0721、0.5219、0.1755mg·g-1生药。结论所建方法操作简便、准确,可作为夏枯草中芦丁和槲皮素的含量测定方法。
Objective To determine the contents of rutin and quercetin in Prunella vulgaris by HPLC. Method The column was Alltima C18 (250 mm × 4.6 mm, 5 μm). The mobile phase consisted of methanol-0.1% glacial acetic acid. The detection wavelength was 350 nm and the flow rate was 1.0 mL · min-1. The column temperature was 30 ℃. Results The linear ranges of rutin and quercetin were 0.0149-0.2380 mg · mL -1 (r = 0.9999) and 0.0019-0.0306 mg · mL -1 (r = 0.9998) respectively. The average recoveries of rutin and quercetin were 97.78% (RSD = 0.83%), 101.18% (RSD = 0.83%). The contents of rutin in the five producing areas of Anhui, Hubei, Guizhou, Jiangxi and Guangxi were 0.3527,0,0.2337,2.1164,0.9578mg · g-1, the contents of quercetin were 0.0872,0.0473,0.0721,0.5219,0.1755mg · g-1 crude drug. Conclusion The method is simple and accurate and can be used as a method for the determination of rutin and quercetin in Prunella vulgaris.