Five strains of antarctic bacteria producing extracellular low-temperature lipase are screened from seawater collected by CTD during the Chinese 18th Antarctic Scientific Expedition. Their phylogenetic positions on the basis of amplification, comparison and analysis of almost complete 16S rDNA sequence are determined by neighbor-joining analysis. Phylogenetic analysis indicates that allof these five strains belong to γ-proteobacteria. The strains 1-1-10-2 and 9-2 are classified as genus Pseudoalteromonas sp. and genus Psychrobacter sp. respectively. The strains 2-5-10-1, 2-2-2-1 and 1-2-8-1 are classified as genus Moritella sp.