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目的建立同时测定琥珀多寐丸中远志(口山)酮Ⅲ、3,6’-二芥子酰基蔗糖、细叶远志皂苷、远志皂苷B、去氢土莫酸和茯苓酸含量的方法。方法采用波长切换检测的高效液相色谱法,色谱柱采用依利特C_(18)色谱柱,流动相为乙腈(A)-体积分数为0.1%的磷酸溶液(B)系统,进行梯度洗脱;0~36 min检测波长为320 nm(检测远志(口山)酮Ⅲ、3,6’-二芥子酰基蔗糖),36~75 min检测波长为210 nm(检测细叶远志皂苷、远志皂苷B、去氢土莫酸、茯苓酸);流速为1.3 mL·min~(-1);柱温为30℃。结果在优化的色谱条件下,远志(口山)酮Ⅲ、3,6’-二芥子酰基蔗糖、细叶远志皂苷、远志皂苷B、去氢土莫酸和茯苓酸分别在6.46~129.20 mg·L~(-1)(r=0.999 2)、6.13~122.60 mg·L~(-1)(r=0.999 5)、13.17~263.40 mg·L~(-1)(r=0.999 9)、14.15~283.00 mg·L~(-1)(r=0.999 6)、5.35~107.00 mg·L~(-1)(r=0.999 7)、4.50~90.00 mg·L~(-1)(r=0.999 3)内浓度与峰面积呈良好的线性关系;6种成分的平均加样回收率(n=6)分别为97.69%、96.87%、100.04%、97.74%、97.67%、99.16%,RSD分别为1.35%、0.96%、0.59%、0.93%、1.02%、1.10%。结论该检测方法可用于琥珀多寐丸中远志(口山)酮Ⅲ、3,6’-二芥子酰基蔗糖、细叶远志皂苷、远志皂苷B、去氢土莫酸和茯苓酸的含量分析。
Objective To establish a method for the simultaneous determination of content of amberpasthuanzhiyuan (Kushan) ketones Ⅲ, 3,6’-di-erucylsucrose, Prunella vulgaris, Tenebrio saponin B, dehydroarteromamide and pachyrinine. Methods High performance liquid chromatography with wavelength - switching detection was used. The column was eluted with elution C18 column and the mobile phase consisted of acetonitrile (A) - 0.1% phosphoric acid solution (B) The detection wavelength was 320 nm at 0-36 min (detection of Polygalacetin III, 3,6’-di-erucyl-sucrose), and the detection wavelength was 210 nm at 36-75 min (detection of Polygalaceae saponins, Hydrophobic acid, Pachyrin acid); the flow rate was 1.3 mL · min -1; the column temperature was 30 ℃. Results Under optimized chromatographic conditions, Polygalacetin, 3,6’-di-erucylsucrose, Prunella vulgaris, Polygonatum saponin B, daptomycin and pachyrin acid were in the range of 6.46-129.20 mg · (R = 0.999 2), 6.13 ~ 122.60 mg · L -1 (r = 0.999 5), 13.17 ~ 263.40 mg · L -1 (r = 0.999 9) and 14.15 ~ 283.00 mg · L -1 (r = 0.999 6), 5.35-107.00 mg · L -1 (r = 0.999 7), 4.50-90.00 mg · L -1 (r = 0.999 3) showed a good linear relationship with the peak area. The average recoveries (n = 6) of the six components were 97.69%, 96.87%, 100.04%, 97.74%, 97.67% and 99.16%, respectively 1.35%, 0.96%, 0.59%, 0.93%, 1.02%, 1.10%. Conclusion The method can be used for the content analysis of amberlips Pill Zhongyuan (Kushan) ketone Ⅲ, 3,6’-arsuccinosylsuccinate, Prunella vulgaris, Tenebrio saponin B, daptomycin and pachyrin acid.