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金黄色葡萄球菌agr系统能够调控多种毒力因子的表达,在该系统中,受体组氨酸蛋白激酶AgrC感受外部信号并传递给细胞内,在整个agr系统中起着重要的作用,成为药物发现的新靶点,该蛋白由agrC基因编码。通过分析发现,agrC基因的保守性非常差,这可能是由于它作为受体需要与信号分子结合的特异性所决定的。通过对已经公开的agrC基因序列进行比对分析,设计了多条引物,以金黄色葡萄球菌ATCC 6538的基因组DNA为模板,进行了PCR扩增并转化大肠埃希菌克隆该基因,获得了金黄色葡萄球菌ATCC 6538附属基因调节系统agrC基因的全序列。同时,通过对现有的提取金黄色葡萄球菌基因组的方法做了改进,得到了一种大量提取了金黄色葡萄球菌DNA的方法,获得的DNA纯度较高。
Staphylococcus aureus agr system can regulate the expression of a variety of virulence factors in the system, the receptor histidine protein kinase AgrC feel the external signal and passed to the cell, in the agr system plays an important role, as New target for drug discovery, encoded by the agrC gene. The analysis revealed that the conservatism of the agrC gene is very poor, probably due to its specificity as a receptor that needs to bind to signaling molecules. By comparing the published sequences of agrC gene, a number of primers were designed and the genomic DNA of S.aureus ATCC 6538 was used as a template for PCR amplification and transformed into Escherichia coli to clone the gene. Gold The complete sequence of the agrC gene of the Staphylococcus aureus ATCC 6538 accessory gene regulatory system. At the same time, the method of extracting the genome of Staphylococcus aureus is improved, and a method for extracting the DNA of Staphylococcus aureus by a large amount is obtained, and the purity of the obtained DNA is high.