建立了以毛细管电泳为基础的测定蛋白激酶Ａ活性的新方法，可作为激酶测活的通用方法．此法基于底物及其磷酸化产物很容易在毛细管电泳中分开，且酶活力可用积分值计算，同时又发展了连续进样技术，能在一次电泳中同时进行１０个以上的酶活性测定，新方法操作简单，灵敏度和精确性均优于常规的同位素法． A new method for the determination of protein kinase A activity based on capillary electrophoresis was established, which can be used as a universal method for kinase activity. This method is based on the substrate and its phosphorylation products are easily separated in the capillary electrophoresis, and the enzyme activity can be calculated using the integral value, but also developed a continuous injection technology, in a single electrophoresis in more than 10 enzyme activity determination, The new method is simple, sensitive and accurate than the conventional isotope method.