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自然感染或实验室感染恶性疟原虫子孢子后均可产生保护性免疫。由自然感染而产生的抗子孢子抗体可识别位于子孢子表面的环子孢子(CS)蛋白。CS蛋白分子含有4～12个氨基酸组成的片段。这些氨基酸是连续重复10～37次。此重复片段可能是抗体识别的靶位点,并已可能通过基因工程或人工合成等方法加以制备。采用世界卫生组织免疫研究和培训中心Giudice G Del博士惠赠的人工合成恶性疟原虫CS蛋白重复序列肽链(天冬酰胺-丙氨酸-天冬酰胺-脯氨酸)_(40),即(NANP)_(40)作抗原,以酶联免疫吸附试验(ELISA)法对55份恶性疟患者血样进行抗CS蛋白抗体的检测。现将结果简述如下。 试验基本按Giudice(1987)方法。(NANP)_(40)
After natural infection or laboratory infection of Plasmodium falciparum sporozoites can produce protective immunity. Anti-sporozoite antibodies produced by natural infection recognize circumsporozoite (CS) proteins located on the surface of sporozoites. CS protein molecules contain 4 to 12 amino acid fragments. These amino acids are consecutively repeated 10 to 37 times. This repeat may be the target of antibody recognition and may have been prepared by genetic engineering or synthetic methods. The artificial synthetic Plasmodium falciparum CS protein repeat peptide chain (Asparagine-Alanine-Asparagine-Pro) _ (40), which is a gift from Dr. Giudice G Del, WHO Center for Immunization Research and Training, ie NANP) _ (40) as antigens, anti-CS protein antibodies were detected in 55 blood samples of patients with P. falciparum by enzyme-linked immunosorbent assay (ELISA). The results are summarized below. The test was performed essentially according to the method of Giudice (1987). (NANP) _ (40)