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以中国樱桃14个自然居群280个个体为材料,采用SSR分子标记技术分析其遗传多样性和遗传结构。结果显示:11对SSR引物共检测到80个等位基因,各引物扩增条带在4~13条之间。基因多样性指数(h)为0.5431~0.7151,Shannon’s信息指数(I)为0.9057~1.4684。分子方差分析(AMOVA)表明,遗传变异主要来自居群内(70.00%),Mantel检验显示总群体的遗传距离和地理距离显著相关(r=0.472,P=0.011)。因此,中国樱桃在居群水平(PPL=100%,h=0.643,I=1.207)和物种水平(PPL=100%,h=0.743,I=1.591)上均具有较高的遗传多样性。
Using 280 individuals from 14 natural populations of Chinese cherry as material, SSR markers were used to analyze their genetic diversity and genetic structure. The results showed that a total of 80 alleles were detected by 11 pairs of SSR primers and the bands amplified by each primer ranged from 4 to 13. The genetic diversity index (h) was 0.5431 ~ 0.7151, Shannon’s information index (I) was 0.9057 ~ 1.4684. Molecular variance analysis (AMOVA) showed that the genetic variation mainly came from the population (70.00%). The Mantel test showed that the genetic distance of the total population was significantly correlated with the geographic distance (r = 0.472, P = 0.011). Therefore, Chinese cherry had high genetic diversity at population level (PPL = 100%, h = 0.643, I = 1.207) and species level (PPL = 100%, h = 0.743, I = 1.591)