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目的掌握抗HIV弱阳性外部对照质控血清的制备方法,有效运用到试验过程中。方法用HIV抗体阴性血清成倍比稀释强阳性血清,采用酶联免疫吸附检验(ELISA)来检测各稀释度OD值,并计算S/CO值来确定某一稀释度为配置稀释度(即临界值2-3倍)。结果采用倍比稀释法,可获得所需外部质控所用血清浓度,并且连续检测20次,绘制出室内质控图。结论制备适合自己试验室的弱阳性的外部质控血清方法简单,使用起来方便,也易于保存,适用于HIV初筛实验室日常检测的室内质量控制工作。
Objective To establish a method for preparing quality control anti-HIV weak positive control serum, and to effectively apply it to the test. Methods Strongly positive sera were diluted by multiplying HIV antibody-negative sera. ELISA was used to detect the OD value of each dilution and the S / CO value was calculated to determine whether a certain dilution was the dilution of configuration Value 2-3 times). Results The ratio dilution method was used to obtain the serum concentrations required for the external controls, and 20 consecutive tests were performed to map the internal quality control. Conclusion The preparation of weakly positive external control serums suitable for our laboratory is simple, convenient to use and easy to store and is suitable for the laboratory quality control of HIV testing laboratories.