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为找外源性的自由基清除剂和抗氧化剂,课题对北极放线菌T-2-3胞外多糖(Arctic Streptomyces sp.extracellular polysaccharide,ASEP)的抗氧化能力进行了研究。通过测定总还原能力(T-AOC)、亚铁离子鳌合能力、超氧阴离子自由基(O2-.)清除能力、羟基自由基(.OH)清除能力和H2O2清除能力,对ASEP和进一步分离组分ASEP-3进行了抗氧化活性初步评价。此外,体外检测了ASEP和ASEP-3对.OH诱导的小鼠肝线粒体MDA生成影响,对H2O2引起的小鼠红细胞溶血的抑制作用及对.OH诱导的肝线粒体肿胀程度的影响。结果显示,ASEP具有一定的抗氧化活性,当ASEP和ASEP-3浓度为200μg/mL时,其对.OH的清除作用分别达到58.0%和99.5%,对O2-.的清除作用达到38.9%和74.2%,对H2O2的清除作用达到12.1%和20.4%,对H2O2引起的氧化溶血的抑制作用达到10.2%和22.8%,对小鼠肝线粒体脂质过氧化的保护作用达到7.9%和14.5%,均呈一定的量效关系,表明ASEP具有一定的抗氧化作用。
In order to find exogenous free radical scavengers and antioxidants, the antioxidant activity of Arctic Streptomyces sp. Extracellular polysaccharide (ASEP) was studied. ASEP was further separated by determining the total reducing ability (T-AOC), ferrous ion chelating ability, superoxide anion radical (O2-) scavenging ability, hydroxyl radical (.OH) scavenging ability and H2O2 scavenging ability The component ASEP-3 was evaluated for its antioxidant activity. In addition, the effect of ASEP and ASEP-3 on the malondialdehyde (MDA) production induced by .OH in mice, the inhibition of hemolysis induced by H2O2 in mice and the degree of hepatic mitochondria swelling induced by .OH were examined in vitro. The results showed that ASEP had a certain antioxidant activity. When ASEP and ASEP-3 were 200μg / mL, their scavenging effects on OH were 58.0% and 99.5%, respectively, and the scavenging effect on O2- was 38.9% and 74.2%, respectively. The scavenging effects on H2O2 reached 12.1% and 20.4%, respectively. The inhibitory effects on H2O2-induced oxidative hemolysis reached 10.2% and 22.8%, respectively. The protective effects on mouse liver mitochondrial lipid peroxidation reached 7.9% and 14.5% Showed a certain dose-effect relationship, indicating that ASEP has a certain antioxidant effect.