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目的探讨四逆汤干预心肌梗死模型代谢组学产物的相互作用及生物学通路。方法从中国知网、万方数据库、维普数据库、Embase、Pubmed等数据库中检索四逆汤干预心肌梗死的代谢组学实验研究;对纳入研究的代谢产物进行汇总;信号通路分析采用KEGG,代谢产物分子注释、相关的酶或转运蛋白及其相关性质分析采用HMDB,代谢产物路径可视化采用Met PA网络软件。结果 3项研究的40种产物被纳入四逆汤干预心肌梗死模型代谢产物谱,Met PA分析显示40种代谢产物主要参与了39条代谢路径。通过通路拓扑分析选出了9条P<0.05的通路,作为四逆汤干预心肌梗死过程的代谢通路,分别是:氨酰t RNA生物合成;柠檬酸循环(TCA循环);缬氨酸、亮氨酸和异亮氨酸的生物合成;丙氨酸、天冬氨酸和谷氨酸代谢;D-谷氨酰胺和谷氨酸代谢;氮代谢;不饱和脂肪酸的生物合成;精氨酸和脯氨酸代谢;半乳糖代谢。结论四逆汤干预大鼠心肌梗死代谢组生物信息学分析显示其作用机制涉及到糖、蛋白质最终共同通路的三羧酸循环、氨基酸合成、转运等方面,体现了四逆汤作用“多因、多效、多靶点”的作用特点。
Objective To investigate the interactions and biological pathways of metabolomics products of Sini decoction in myocardial infarction model. Methods Metabolomics of Sini Decoction for myocardial infarction was retrieved from databases such as CNKI, Wanfang Database, VIP database, Embase, Pubmed and so on. The metabolites included in the study were summarized. The signal pathways were analyzed by KEGG, metabolites Molecular annotations, related enzymes or transporters and their associated properties were analyzed using HMDB and metabolite pathways were visualized using Met PA web-based software. Results 40 products from 3 studies were included in the metabolite profile of Sini Decoction intervention myocardial infarction model. Met PA analysis showed that 40 metabolites mainly involved in 39 metabolic pathways. Nine pathways of P <0.05 were selected as the metabolic pathways for the intervention of Sini decoction in myocardial infarction. Pathways were: aminoacyl-tRNA biosynthesis; citrate cycle (TCA cycle); valine Alanine, aspartate and glutamate metabolism; D-glutamine and glutamate metabolism; nitrogen metabolism; unsaturated fatty acid biosynthesis; arginine and isoleucine Proline metabolism; Galactose metabolism. Conclusion The bioinformatics analysis of Sini decoction in myocardial metabolism in rats shows that its mechanism of action involves the tricarboxylic acid cycle, amino acid synthesis and transport in the final common pathway of sugar and protein, which reflects the effect of Sini Decoction , Multi-effect, multi-target "role characteristics.