目的观察尾吊模拟失重后大鼠比目鱼肌中ERK1/2磷酸化状态的变化。方法采用尾部悬吊大鼠模拟失重效应,以Westernblot技术检测游离的大鼠比目鱼肌中总的ERK1/2含量和其磷酸化状态的变化。结果7d和14d尾吊模拟失重后,大鼠比目鱼肌内总ERK1/2的含量没有发生改变。而ERK1磷酸化状态在7d和14d模拟失重后均显著降低。ERK2的磷酸化状态在尾吊7d后显著下降,而在14d尾吊后其下降程度却未达显著性。结论尾吊模拟失重后可降低大鼠比目鱼肌内ERK1/2的磷酸化状态。 Objective To observe the changes of phosphorylation of ERK1 / 2 in soleus after simulated tail loss in rats. Methods The tail suspension rats were used to simulate the effects of weightlessness. The total ERK1 / 2 and the phosphorylation status in the soleus were measured by Western blot. Results The total ERK1 / 2 level in the soleus muscle of rats did not change after simulated weightlessness on the 7th and 14th tail suspensions. The ERK1 phosphorylation state after 7d and 14d simulated weightlessness were significantly reduced. The phosphorylation status of ERK2 decreased significantly after 7 days, but it did not reach significance after 14 days tail suspension. CONCLUSIONS: The tail-hang simulation can decrease the phosphorylation of ERK1 / 2 in the soleus after simulated weightlessness.