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目的 探讨在胃癌组织中 Ｐ Ｔ Ｅ Ｎ 基因和蛋白的表达情况及临床病理意义。方法 应用免疫组织化学和原位杂交技术检测５０ 例胃癌及其癌旁组织中 Ｐ Ｔ Ｅ Ｎ 蛋白及 Ｐ Ｔ Ｅ Ｎ ｍ Ｒ Ｎ Ａ 表达情况，同时结合病人的临床病理资料进行分析。结果 ５０ 胃癌组织中， Ｐ Ｔ Ｅ Ｎ 蛋白阳性表达率４８ ％ ，阳性率５２ ％ ，阳性反应分布在胞浆中。５０ 例胃癌组织中 Ｐ Ｔ Ｅ Ｎ ｍ Ｒ Ｎ Ａ 阳性表达率６０ ％ ，阳性率４０ ％ ，阳性信号显示于胞浆中。 Ｐ Ｔ Ｅ Ｎ 蛋白及 Ｐ Ｔ Ｅ Ｎ ｍ Ｒ Ｎ Ａ 在胃癌组织中的阳性表达与患者性别，年龄无明显相关性，但均与组织分化程度明显相关，高分化腺癌组的阳性率分别为７７ ．８ ％ 及８８ ．９ ％ ，低分化腺癌组的阳性率分别为３１ ．３ ％ 及４３ ．８ ％ 。结论 胃癌组织中存在较高比例的 Ｐ Ｔ Ｅ Ｎｍ Ｒ Ｎ Ａ 及 Ｐ Ｔ Ｅ Ｎ 蛋白阳性表达，说明在胃癌的发生发展中， Ｐ Ｔ Ｅ Ｎ 基因失活起着重要作用，两者的阳性表达均有一定的预后意义。
Objective To investigate the expression and clinicopathological significance of P T E N gene and protein in gastric cancer. Methods Immunohistochemistry and in situ hybridization were used to detect the expression of P T E N protein and P T E N m R N A in 50 cases of gastric cancer and its adjacent tissues. The clinical and pathological data of the patients were analyzed. Results In gastric cancer tissues, the positive expression rate of P T E N protein was 48%, and the positive rate was 52%. The positive reaction was distributed in the cytoplasm. The positive expression rate of P T E N m R N A in 60 cases of gastric cancer was 60%, and the positive rate was 40%. The positive signal was shown in the cytoplasm. The positive expression of P T E N protein and P T E N m R N A in gastric cancer was not significantly correlated with the sex and age of the patients, but all had significant correlation with the degree of histological differentiation. The positive rates of the well-differentiated adenocarcinoma group were 77. 8 % and 88. The positive rate of 9 %, poorly differentiated adenocarcinoma group was 31. 3% and 43. 8 %. Conclusion There is a high proportion of positive expression of P T E Nm R N A and P T E N protein in gastric cancer tissues, indicating that P T E N gene inactivation plays an important role in the development of gastric cancer. All have a certain prognostic significance.