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目的:观察北京市人群p38 MAPK基因多态性与糖尿病前期发病风险及胰岛B细胞功能改变的相关性。方法:从2007年及2009年北京市3个大型社区糖尿病流行病学调查的数据库中,选取临床资料完整且无亲缘关系的受试者1466例,经口服糖耐量试验证实为糖尿病前期组845例,口服糖耐量试验结果正常且无糖尿病家族史的正常对照组621例。应用质谱法对p38MAPK基因的3个单体型标记单核苷酸多态性(SNPs)rs2072878(A>G)、rs2076139(C>T)及rs742186(T>C)进行等位基因及基因型检测。结果:p38MAPK基因rs2072878位点等位基因A携带者患糖尿病前期风险显著升高(OR=1.295,P<0.05),基因型AA携带者较基因型AG/GG携带者发生糖尿病前期的风险显著升高(OR=1.346,P<0.05),校正性别、年龄、血压、体质指数及腹围后差异更显著(OR′=1.442,P′<0.05)。rs2072878与rs2076139组成的单体型GC发生糖尿病前期风险显著降低(OR=0.763,P<0.05)。进一步按照年龄分层分析,结果显示,年龄≥60岁人群SNP rs2072878的基因型与糖尿病前期发病风险显著相关,基因型AA的受试者较基因型AG/GG的受试者发生糖尿病前期的风险升高1.429倍;并且,这种风险的升高在调整年龄、性别、血压、体质指数及腹围后更加显著(OR′=1.630,P′<0.05)。以年龄为连续变量,对风险SNP与年龄对糖尿病前期风险的作用做回归分析显示,年龄和rs 2072878存在显著的交互作用(P<0.05)。结论:北京人群中p38MAPK基因多态性与糖尿病前期发病风险存在相关性,p38SNP rs2072878对糖尿病前期发病风险的作用在年龄≥60岁的老年人群更加显著。
Objective: To observe the association between the p38 MAPK gene polymorphism and the risk of pre-diabetes and changes of islet B cell function in Beijing population. Methods: From the data of 3 large-scale community diabetes epidemiological survey in Beijing in 2007 and 2009, 1466 subjects with complete and unrelated clinical data were selected and 845 pre-diabetes patients were confirmed by oral glucose tolerance test , Oral glucose tolerance test results were normal and no family history of diabetes in the control group 621 cases. The alleles and genotypes of three haplotype-labeled single nucleotide polymorphisms (SNPs) rs2072878 (A> G), rs2076139 (C> T) and rs742186 (T> C) Detection. Results: The allele A of rs2072878 in p38MAPK gene had a significantly increased risk of pre-diabetes (OR = 1.295, P <0.05). The risk of pre-diabetes in genotype AA carriers was significantly higher than that of carriers of genotype AG / GG (OR = 1.346, P <0.05). The adjusted sex, age, blood pressure, body mass index and abdominal circumference were more significant (OR ’= 1.442, P <0.05). The haplotype GC of rs2072878 and rs2076139 had a significantly lower risk of pre-diabetes (OR = 0.763, P <0.05). Further stratified by age, the results showed that genotypes of SNP rs2072878 were significantly associated with pre-diabetes risk in people over 60 years of age, and subjects with genotype AA had a pre-diabetes risk than those with genotype AG / GG 1.429 times higher than those in the control group. The risk increased more significantly after adjusted for age, sex, blood pressure, body mass index and abdominal circumference (OR ’= 1.630, P <0.05). Using age as a continuous variable, regression analysis of the role of risk SNP and age in the risk of pre-diabetes showed a significant interaction between age and rs 2072878 (P <0.05). Conclusion: There is a correlation between the p38MAPK gene polymorphism and the risk of pre-diabetes in Beijing population. The effect of p38SNP rs2072878 on the risk of pre-diabetes is more significant in the elderly population over 60 years old.