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本文观察比较了博莱霉素A_5(国产)、博莱霉素A_2B_2(日本)和博莱霉素A_2B_2国际标准品对于人体胃癌细胞的总脱氧核糖核酸和其50kb部分,以及人舌癌细胞的总脱氧核糖核酸的体外断链效应。作用后脱氧核糖核酸经琼脂糖微型快速电泳和溴化乙锭染色后,紫外荧光摄影。显示三种博莱霉素产品对人癌细胞双链脱氧核糖核酸的作用模型电泳图。并观察了作用时间及药物浓度的效应。人体DNA10μg在博莱霉素400μg/ml(～0.27mM)浓度下,37℃作用30分钟后,即可观察到人体DNA的断裂效应;37℃作用120分钟后,几乎全部人体DNA分子均被切割断链。人体DNA20μg在系列浓度的博莱霉素存在下,37℃作用120分钟,观察到博莱霉素断链的最低有效浓度为1μg/ml(～0.67μM)左右。没有发现博莱霉素-人胃癌细胞脱氧核糖核酸与博莱霉素-人舌癌细胞脱氧核糖核酸的作用模型之间有何明显差别。 本文还讨论了以人体细胞脱氧核糖核酸为对象,研究抗癌药物作用模式的实践意义,和本实验系统正被应用于人体“癌基因”(Oncogenes)有关研究的价值。
In this paper, we compared the total deoxyribonucleic acid (DNA) and its 50kb portion of human gastric cancer cells with bleomycin A 5 (domestic), bleomycin A 2 B 2 (Japan) and bleomycin A 2 B 2 international standard, In vitro chain scission of DNA. After the effect of DNA by agarose micro-fast electrophoresis and ethidium bromide staining, UV fluorescence photography. Shows three bleomycin products on human cancer cells double-stranded DNA model electrophoresis model. And observed the effect of time and drug concentration. Human DNA 10μg At the concentration of 400μg / ml (~0.27mM) of bleomycin, the effect of DNA cleavage was observed at 37 ° C for 30 minutes. Almost all human DNA molecules were cleaved after 120 minutes at 37 ° C Broken chain. In the presence of serial concentrations of bleomycin, human body DNA 20 μg was allowed to act at 37 ° C for 120 minutes and the lowest effective concentration of bleomycin chain scission was observed to be about 1 μg / ml (~0.67 μM). No clear difference was observed between the model of bleomycin-human gastric cancer cell DNA and the model of bleomycin-human tongue cancer cell deoxyribonucleic acid. This article also discusses the practical significance of using human cytosine deoxyribonucleic acid as a target to study the mode of action of anticancer drugs and the value of the experimental system being applied to oncogenes in human body.