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目的研究组蛋白H2AX在高氧致肺损伤新生大鼠肺组织中的表达,并探讨其在高氧肺损伤中的作用。方法将80例新生大鼠随机分为实验组(高氧组)和对照组(空气组),建立高氧致新生大鼠肺损伤的模型,在实验开始后1、2、3、5、7天取肺组织标本,运用免疫荧光技术和Western-blot方法检测高氧肺损伤过程中H2AX的表达规律。结果实验组大鼠肺组织H2AX蛋白平均荧光强度在实验开始后第1、2、3天(P<0.01)和第5天(P<0.05)明显高于对照组,第7天与对照组无明显差异(P>0.05);实验组H2AX蛋白平均荧光强度呈逐渐下降趋势(F=45.02,P<0.01),在第7天时接近对照组水平。H2AX蛋白相对表达量变化趋势与免疫荧光法结果相同,实验开始后第1天明显升高,并随着暴露高氧时间的延长逐渐降低。结论 H2AX可能与高氧致急性肺损伤密切相关。
Objective To investigate the expression of histone H2AX in lung tissue of neonatal rats with hyperoxia-induced lung injury and to explore its role in hyperoxia-induced lung injury. Methods Eighty newborn rats were randomly divided into experimental group (hyperoxia group) and control group (air group) to establish a model of lung injury induced by hyperoxia in neonatal rats. After the start of the experiment, 1,2,3,5,7 Lung tissue samples were taken day by day, and the expression of H2AX in hyperoxia-induced lung injury was detected by immunofluorescence and Western-blot. Results The mean fluorescence intensity of H2AX protein in the lung tissue of the experimental group was significantly higher than that of the control group on the 1st, 2nd and 3rd days (P <0.01) and on the fifth day (P <0.05) (P> 0.05). The mean fluorescence intensity of H2AX decreased gradually in the experimental group (F = 45.02, P <0.01), and reached the level of the control group on the 7th day. The trend of relative expression of H2AX protein was the same as that of immunofluorescence method. On the first day after the start of the experiment, the expression of H2AX protein was significantly increased, and gradually decreased with the prolongation of exposure to hyperoxia. Conclusions H2AX may be closely related to hyperoxia-induced acute lung injury.