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为了培育牙鲆(Paralichthys olivaceus)抗鳗弧菌(Vibrio anguillarum)病的品系或品种,2009和2012年利用中国牙鲆抗病群体、从日本和韩国引进的牙鲆群体以及2007年和2009年从大量牙鲆家系中选留的优良家系为亲本,通过巢式杂交、三元杂交及雌核发育等方法,分别于2009年和2012年建成牙鲆家系43个和65个,选取2009年的33个家系和2012年的43个家系进行鳗弧菌感染实验,共筛选出13个抗病家系,其中3个家系的存活率极显著高于对照组(P<0.01),另外10个家系的存活率显著高于对照组(P<0.05),这13个家系包含F3家系、雌核发育一代和二代家系各1个,F2家系3个,在以上6个家系中,除了1个F2家系,其他家系的亲本均来自于抗病家系且鳗弧菌感染存活率的变异系数都低于10%。对连续三代抗病家系进行分析,发现在上述13个抗病家系和2007年筛选出的3个抗病家系共16个抗病家系中有13个家系来自于中国牙鲆抗病群体相关,结果表明,部分F1、F2、F3和雌核发育家系较好地遗传了其亲本的抗病性能,抗病性能稳定,为培育抗鳗弧菌病的牙鲆品系或品种奠定了基础。
In order to cultivate the strains or varieties resistant to Paralichthys olivaceus against Vibrio anguillarum, in 2009 and 2012, the Chinese flounder flounder population, the Japanese flounder populations introduced from Japan and South Korea, A large number of flounder family selected excellent family as the parent, by means of nested hybridization, three-way cross and gynogenetic development, respectively, in 2009 and 2012, flounder Pai family of 43 and 65, select 2009, 33 Thirteen families were tested for V. anguillarum infection in 2012, and 13 resistant families were screened. The survival rates of three families were significantly higher than those of the control group (P <0.01). The survival rates of the other ten families (P <0.05). These 13 families included F3, one gynogenetic and two second-generation pedigrees, and three F2 families. Of the six pedigrees, except for one F2 pedigree, others The parents of the pedigrees were all from the resistant pedigree and the coefficient of variation (CV) of Vibrio anguillarum infection survival rate was less than 10%. Analysis of three generations of resistance pedigrees showed that among the 13 resistance families and 13 resistance families selected in 2007, 13 of the 16 resistance families originated from the Chinese flounder disease-resistant population and the results The results showed that some F1, F2, F3 and gynogenetic lineages inherited their parents’ disease resistance well and their resistance to disease was stable, which laid the foundation for cultivating flounder strains or varieties resistant to Vibrio anguillarum.