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目的建立同时测定栀子金花丸中绿原酸、栀子苷、黄芩苷、小檗碱、汉黄芩苷、黄芩素、芦荟大黄素、汉黄芩素、大黄素、大黄酚、大黄素甲醚11种成分的UPLC法。方法采用UPLC法,Acquity UPLC BEH C18色谱柱(100 mm×2.1 mm,1.7μm);乙腈(A)-0.1%磷酸水溶液(B)为流动相,梯度洗脱;体积流量为0.4 mL/min;柱温35℃;检测波长:绿原酸为324 nm,栀子苷为238 nm,小檗碱为345 nm,黄芩苷、汉黄芩苷、黄芩素、汉黄芩素为276 nm,芦荟大黄素、大黄素、大黄酚、大黄素甲醚为254 nm。结果被测定的11种成分色谱峰均有良好的分离度,方法精密度、重复性的RSD均小于2%;在室温条件下24 h内稳定;各成分均有较宽的线性范围和良好的线性关系(r≥0.999 5);平均加样回收率98.68%~100.47%,RSD均<1.5%。结论本方法操作简便,测定结果准确可靠,可用于栀子金花丸的质量控制。
Objective To establish a method for simultaneous determination of chlorogenic acid, geniposide, baicalin, berberine, baicalin, baicalein, aloe-emodin, wogonin, emodin, chrysophanol, Eleven components of the UPLC method. Methods UPLC was performed on an Acquity UPLC BEH C18 column (100 mm × 2.1 mm, 1.7 μm). Acetonitrile (A) -0.1% phosphoric acid solution (B) was used as mobile phase with gradient elution. The volume flow rate was 0.4 mL / min. The column temperature was 35 ℃. The detection wavelength was 324 nm for chlorogenic acid, 238 nm for jasminoidin and 345 nm for berberine. The concentrations of baicalin, baicalin, baicalein and wogonin were 276 nm, Emodin, chrysophanol, physcion is 254 nm. Results The chromatographic peaks of all the 11 components showed good resolution. The precision and reproducibility of the methods were all less than 2%. The RSDs were stable within 24 hours at room temperature. Each component had a wide linear range and a good Linear relationship (r≥0.999 5); average recovery rate of 98.68% ~ 100.47%, RSD <1.5%. Conclusion The method is simple, accurate and reliable, and can be used for quality control of Gardenia jinhuahua pill.