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2002年采用PCR-核酸探针斑点杂交法检测了乳山对虾养殖场1000余份样品携带白斑综合征病毒(WSSV)的情况。结果显示,639例对虾样品中阳性检出率为26·6%;77例蟹类样品中阳性检出率为18·2%;266例浮游动物样品中阳性检出率为38·3%,3~9月份浮游动物阳性率呈下降趋势,消毒后水体中浮游动物的阳性率仍很高;30例贝类样品检测均为阴性;204例底泥样品中,阳性检出率为17·6%,22例抽滤海水样品检测均为阴性。结果表明,虾、蟹类在传播WSSV中起着重要作用,贝类、海水传播WSSV的可能性很小,浮游动物、底泥在传播WSSV中的作用和机制应引起高度重视。
In 2002, PCR-nucleic acid probe hybridization was used to detect WSSV infection in more than 1000 samples of the Rushan shrimp farm. The results showed that the positive detection rate was 26.6% in 639 samples of shrimp and 18.2% in 77 samples of crabs. The positive detection rate was 38.3% in 266 zooplankton samples, The positive rate of zooplankton decreased from March to September, and the positive rate of zooplankton in the water after disinfection was still high. The detection rate of all 30 shellfish samples was negative. In 204 sediment samples, the positive rate was 17.6 %, 22 samples of seawater filtration test were negative. The results showed that shrimp and crabs play an important role in the spread of WSSV. Shellfish and seawater are less likely to spread WSSV. The role and mechanism of zooplankton and sediment in WSSV transmission should be given high priority.