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在静置培养条件下,对粉被虫草cp菌株产N6‐(2‐羟乙基)腺苷(简称HEA)的培养基及其组分进行优化。采用单因素实验方法,以HPLC法对cp菌丝体中HEA进行检测,以HEA的产量为指标,结果表明查氏培养基有利于cp菌株产HEA,其产量是沙氏和PD培养基的3.7倍和4.48倍。以查氏培养基为基础培养基,进行碳、氮源的筛选中,最有利于cp菌株产HEA的是蔗糖和硝酸钠;在无机盐的筛选中,K2HPO4是促进cp菌株累积HEA最主要的无机盐,其HEA产量较CK增长了5 822.37%(提高了58.2倍);在查氏培养基上添加不同前体物和氨基酸,发现其结构类似物腺苷、次黄嘌呤和腺嘌呤都有促进cp菌株累积HEA的能力,其中腺苷和次黄嘌呤能提高其产量60%以上;而组氨酸是最有利于cp菌株累积HEA的氨基酸,产量达到(45.56±2.8)mg/L,较CK提高HEA产量251.68%,其次是L‐谷氨酸增产184.19%。
Under static culture conditions, the medium and components of N6- (2-hydroxyethyl) adenosine (abbreviated as HEA) powder produced by Cordyceps cp strain were optimized. The single factor experimental method was used to detect the HEA in cp mycelia by HPLC, and the yield of HEA was used as an index. The results showed that the Charcter culture medium was good for the production of HEA by cp strain, and its yield was 3.7% of Shas and PD medium Times and 4.48 times. Among them, K2HPO4 is the most important factor to promote the accumulation of HEA in cp strains in screening of inorganic salts, which is most conducive to the screening of carbon and nitrogen sources. Inorganic salts, its HEA yield increased by 5 822.37% compared with CK (58.2-fold increase); different precursors and amino acids were added to the culture medium of C.subtilis, and the structural analogs of adenosine, hypoxanthine and adenine were found The results showed that adenosine and hypoxanthine could increase the yield of HEA by more than 60%, while histidine was the most effective amino acid for HEA accumulating in cp strain with a yield of (45.56 ± 2.8) mg / L CK increased the yield of HEA by 251.68%, followed by L-glutamic acid by 184.19%.