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探讨新疆维吾尔族子宫颈鳞癌发生中ＨＰＶ１６／１８、ｐ５３、ＰＣＮＡ三因素的作用及相关性。方法：应用ＰＣＲ和免疫组化法检测１２７例维吾尔族患者宫颈组织的ＨＰＶ１６／１８ＤＮＡ和ｐ５３蛋白、ＰＣＮＡ的表达。结果：ＨＰＶ１６／１８在正常宫颈、宫颈上皮内瘤样病变（ＣＩＮ）、宫颈鳞癌中检出率分别为１２．５％、２２．５％和５０．０％；ｐ５３、ＰＣＮＡ在三组阳性表达率分别为６．３％、２２．５％、５４．７％和６．３％、６２．８％、８１．２％。统计学分析表明，ｐ５３、ＰＣＮＡ在子宫颈癌与非癌组织中有显著性差异（Ｐ＜０．０１）。 ｐ５３蛋白、 ＰＣＮＡ表达与宫颈鳞癌临床分期、组织学分级、肿瘤大小无关（Ｐ＞０．０５）。有淋巴结转移的５例患者，３例为ＨＰＶ１６／１８、ｐ５３、ＰＣＮＡ三项均阳性。结论：ＨＰＶ１６／１８、ｐ５３、ＰＣＮＡ与子宫颈癌发生均有密切关系，可以作为子宫颈癌早期诊断及判断预后的分子生物学指标。
To investigate the role and correlation of HPV16 / 18, p53 and PCNA in Uygur cervical squamous cell carcinoma in Xinjiang. Methods: The HPV16 / 18 DNA, p53 protein and PCNA expression in 127 cases of uterine cervix were detected by PCR and immunohistochemistry. Results: The positive rates of HPV16 / 18 in normal cervix, cervical intraepithelial neoplasia (CIN) and cervical squamous cell carcinoma were 12.5%, 22.5% and 50.0% respectively. The positive rates of p53 and PCNA in three groups were The expression rates were 6.3%, 22.5%, 54.7% and 6.3%, 62.8% and 81.2% respectively. Statistical analysis showed that there was a significant difference between p53 and PCNA in cervical cancer and non-cancerous tissues (P <0.01). The expression of p53 protein and PCNA had no correlation with clinical stage, histological grade and tumor size (P> 0.05). Five patients with lymph node metastasis, three cases of HPV16 / 18, p53, PCNA were three positive. Conclusion: HPV16 / 18, p53 and PCNA are closely related to the occurrence of cervical cancer, which may serve as a molecular biological indicator for early diagnosis and prognosis of cervical cancer.