论文部分内容阅读
葡萄糖的检验在临床、食品等方面都有重要意义。普遍使用的碱性硫酸铜氧化葡萄糖与色素基团结合的显色法,仍存在一些缺点。最近发展的使用可溶酶法,由于制剂价格较高,方法受到一定的限制。前人曾以多种原理制备过葡萄糖氧化酶电极。这类酶电极具有很好的专一性,使用简便、经济,并可改装成自动分析装置。我们采用市售国产溶氧测定仪与固定化葡萄糖氧化酶明胶膜相配合,构成一套葡萄糖分析仪。其主要原理为:葡萄糖氧化酶能
Glucose test in clinical, food and other aspects of great significance. Commonly used alkaline copper sulfate oxidation of glucose and pigment groups combined color method, there are still some disadvantages. The recent development of the use of soluble enzyme method, due to the higher price of the preparation, the method is subject to certain restrictions. Predecessors have been based on a variety of principles of glucose oxidase electrodes. This kind of enzyme electrode has very good specificity, easy to use, economical, and can be converted into automatic analysis device. We use a commercially available domestic dissolved oxygen analyzer and immobilized glucose oxidase gelatin film to form a glucose analyzer. The main principle is: glucose oxidase can