论文部分内容阅读
利用四氯化碳（CCl4）造成肝细胞脂质过氧化损伤，测定原代培养人胚肝细胞匀浆中羟脯氨酸（Hyp）含量及培养液中Ⅲ型前胶原（PCⅢ）、脂质过氧化物（MDA）、谷胱甘肽过氧化物酶（GSH－Px）活性水平．观察丹参对GSH－Px／MDA比值的影响及其与PCⅢ、Hyp水平的关系。结果：与损伤模型组比较，丹参（1mg／ml）预作用4h，可明显减少受损肝细胞产生PCⅢ、Hyp及MDA，提高GSH－Px活性，GSH－Px／MDA比值增加非常显著（P＜0．01）。提示丹参可抑制CCl4所致人胚肝细胞胶原合成。
Hepatocyte lipid peroxidation injury was induced by carbon tetrachloride (CCl4), and the content of hydroxyproline (Hyp) in primary cultured human embryonic hepatocyte homogenate and type III procollagen (PCIII) and lipid in culture medium were measured. Peroxide (MDA), glutathione peroxidase (GSH-Px) activity levels. Observe the effect of Salvia miltiorrhiza on GSH-Px/MDA ratio and its relationship with PCIII and Hyp levels. Results:Compared with the injury model group, pretreatment with Salviae miltiorrhiza (1 mg/ml) for 4 h could significantly reduce the production of PCIII, Hyp and MDA in damaged hepatocytes, increase the activity of GSH-Px, and increase the ratio of GSH-Px/MDA significantly (P< 0.01). It is suggested that salvia miltiorrhiza can inhibit the collagen synthesis of human embryonic hepatocytes induced by CCl4.