论文部分内容阅读
目的了解乙型肝炎病毒感染者血清中基质金属蛋白酶组织抑制物(TIMP-1和TIMP-2)与透明质酸(HA)、Ⅲ型前胶原(PCⅢ)、Ⅳ型胶原(CⅣ)和层粘连蛋白(LN)的相互关系及临床意义。方法2007年2～12月对498例HBV感染者和100名正常对照者采用双抗体夹心酶联免疫吸附方法(ELISA)测定血清TIMP-1、TIMP-2,放射免疫分析法检测HA、PCⅢ、CⅣ和LN和全自动酶法测定丙氨酸转氨酶(ALT)。结果与健康正常人对照比较,HBV感染者五项指标均有不同程度的升高,除慢性HBV携带者PCⅢ、CⅣ、LN、ALT,慢性肝炎轻度组CⅣ含量水平与对照组差异无统计学意义外,其余各组均与对照组差异有统计学意义(P<0.05)。其中唯有TIMP-1、TIMP-2血清含量从急性肝炎至肝硬化依次进行性升高,慢性HBV携带者组与急性肝炎组相比差异有统计学意义(P<0.01)。血清TIMP-1、TIMP-2与HA、PCⅢ、CⅣ和LN呈显著正相关(P<0.05),与ALT呈正相关(P<0.05)。结论六项指标均可不同程度地反映肝脏的纤维化程度,其中以血清TIMP-1和TIMP-2更为可靠、有效。
Objective To investigate the relationship between TIMP-1, TIMP-2 and the levels of serum hyaluronic acid (HA), PC Ⅲ, collagen Ⅳ (Ⅳ) and lamina propria in patients with hepatitis B virus infection Protein (LN) and its clinical significance. Methods Serum TIMP-1 and TIMP-2 levels were measured by double antibody sandwich enzyme-linked immunosorbent assay (ELISA) in 498 HBV infected persons and 100 normal controls from February 2007 to December 2007. Radioimmunoassay was used to detect the expression of HA, PCⅢ, CⅣ and LN and the determination of alanine aminotransferase (ALT) by the enzymatic method. Results Compared with healthy controls, all five indicators of HBV infection increased in varying degrees. There was no significant difference in the level of CⅣ in PCⅢ, CⅣ, LN, ALT, and mild chronic hepatitis among patients with chronic HBV carriers Significance, the remaining groups were significantly different from the control group (P <0.05). Among them, the serum levels of TIMP-1 and TIMP-2 increased gradually from acute hepatitis to cirrhosis, and there was significant difference between chronic HBV carriers and acute hepatitis group (P <0.01). The serum levels of TIMP-1 and TIMP-2 were positively correlated with HA, PCⅢ, CⅣ and LN (P <0.05), and positively correlated with ALT (P <0.05). Conclusion All six indexes can reflect the degree of liver fibrosis in varying degrees, of which the serum TIMP-1 and TIMP-2 are more reliable and effective.