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目的研究枸杞子提取物对自然衰老小鼠学习记忆影响及其机制。方法 60只12月龄雄性昆明小鼠,随机分4组:老年对照、枸杞子提取物高、中、低剂量组;另15只2月龄小鼠为成年对照组;0.1 ml/10 g灌胃给药17周;Morris迷宫测小鼠学习记忆能力,ELISA法测其海马毒蕈碱型乙酰胆碱受体M1(muscarinic acetylcholine receptor M1,M1R)含量。结果高、中、低剂量组定位航行第5 d潜伏期(37.51±9.47)、(38.13±11.81)、(41.07±8.85)s较老年对照(44.81±7.84)s明显缩短(P<0.05);3个给药组空间探索靶象限留时百分比(38.75±5.35)、(36.34±7.62)、(31.65±6.19)%及过台次数(3.89±0.47)、(3.51±0.28)、(3.09±0.63)次较老年对照(28.82±3.18)%,(2.13±0.32)次明显改善(P<0.05);3个给药组M1R浓度(1 325.81±327.76)、(1 219.35±307.49)、(1 137.84±339.63)pg/m L较老年对照(976.42±321.37)pg/m L明显提高(P<0.01)。结论枸杞子能改善衰老小鼠学习记忆能力并提高其海马内M1受体含量。
Objective To study the effects of Lycium barbarum extract on learning and memory in naturally aging mice and its mechanism. Methods Sixty male Kunming mice, aged 12 months, were randomly divided into 4 groups: aged control, medlar extract high, medium and low dose groups; another 15 2-month-old mice as adult control group; 0.1 ml / The stomach was administered for 17 weeks. Morris maze was used to measure the learning and memory ability in mice. The content of muscarinic acetylcholine receptor M1 (M1R) in hippocampus was measured by ELISA. Results Compared with the control group (44.81 ± 7.84) s, the latency of the 5th, 5th, and 45th days of navigation in the high, middle and low dose groups was significantly shorter (P <0.05) than that in the aged controls (37.51 ± 9.47), (38.13 ± 11.81) and (41.07 ± 8.85) The percentage of target quadrants (38.75 ± 5.35), (36.34 ± 7.62), (31.65 ± 6.19)% and 3.89 ± 0.47 (3.51 ± 0.28) and (3.09 ± 0.63) (28.82 ± 3.18)%, (2.13 ± 0.32) times in the elderly group were significantly improved (P <0.05). The M1R concentration in the three administration groups was (1 325.81 ± 327.76), (1 219.35 ± 307.49), (1 137.84 ± 339.63) pg / m L was significantly higher than that of aged control group (976.42 ± 321.37) pg / m L (P <0.01). Conclusion Fructus Lycii can improve the ability of learning and memory of senile mice and increase the content of M1 receptor in hippocampus.