目的　对益气活血方药活性部位中的芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚进行含量测定。方法　采用高效液相色谱法 ,Diamonsil C18分析柱 2 5 0mm× 4 6mm 5 μm ,流动相 :甲醇- 0 1%磷酸 (87∶13) ,检测波长 :4 36nm ,流速 :1 0ml min ,柱温 :室温。结果　芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚的平均回收率分别为 98 30 %、97 12 %、98 18%、97 6 6 %、97 16 %、RSD分别为 1 6 4%、1 15 %、0 95 %、1 5 1%、1 6 4% (n =5 )。结论　方法操作简便 ,准确 ,重现性好 ,可以用于该活性部位的质量分析。 Objective To determine the contents of aloe-emodin, rhein, emodin, chrysophanol, and emodin in the active parts of Qi-enriching and blood-activating herbs. Methods High performance liquid chromatography was used. Diamonsil C18 column was 250 mm × 4 6 mm 5 μm. Mobile phase: methanol - 0 1% phosphoric acid (87:13). Detection wavelength: 4 36 nm, flow rate: 1.0 ml min, column temperature. : Room temperature. Results The average recoveries of aloe-emodin, rhein, emodin, chrysophanol, and emodin were 98 30%, 97 12%, 98 18%, 97 66%, 97 16%, respectively, and the RSD was 1 6 4%, 1 15%, 0 95%, 1 5 1%, and 164% (n = 5). Conclusion The method is simple, accurate, and reproducible. It can be used for quality analysis of the active site.