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本实验通过观察慢性复合应激对成年大鼠海马NeuroD表达的影响,探讨慢性复合应激与海马神经发生的关系。将成年雄性大鼠32只随机分为慢性复合应激组(简称应激组)和正常对照组(简称对照组)。应激组动物每天不规律交替暴露于四种复合应激原中,共持续6周。然后运用免疫组织化学染色、Western-blot和RT-PCR技术分别观察海马内NeuroD阳性神经元数量、NeuroD蛋白水平和核酸水平的变化。结果显示:慢性复合应激组动物海马齿状回NeuroD阳性神经元数量明显增多(P<0.05);海马NeuroD蛋白的表达明显增强(P<0.05);海马NeuroD mRNA水平明显上调(P<0.05)。表明慢性复合应激可引起海马NeuroD阳性神经元数量增多和NeuroD表达水平升高,提示慢性复合应激可能促进大鼠海马的神经发生。
The experiment by observing chronic complex stress on hippocampal NeuroD expression in adult rats, chronic stress and the relationship between hippocampal neurogenesis. 32 adult male rats were randomly divided into chronic stress group (stress group) and normal control group (referred to as control group). The stress group animals were exposed alternately to four kinds of compound stressors irregularly daily for 6 weeks. The changes of NeuroD positive neurons, NeuroD protein and nucleic acid in the hippocampus were observed by immunohistochemical staining, Western-blot and RT-PCR respectively. The results showed that NeuroD positive neurons in hippocampal dentate gyrus increased significantly (P <0.05), NeuroD protein expression in hippocampus significantly increased (P <0.05), hippocampus NeuroD mRNA level increased significantly (P <0.05) . Chronic stress could induce the increase of NeuroD positive neurons and the increase of NeuroD expression in hippocampus, suggesting that chronic complex stress may promote the neurogenesis of hippocampus in rats.