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【目的】探讨β-细辛醚对抑郁症大鼠海马区细胞的影响。【方法】将80只SD成熟雄性大鼠随机分为4组,即正常组、模型组、氟西汀组(剂量为1.2 mg/kg)、β-细辛醚组(剂量为25 mg/kg)。除正常组外,其他3组复制抑郁症大鼠模型后分别给药,21 d后,采用流式细胞术及TUNEL法,分别对各组大鼠进行凋亡细胞的数量及形态学的检测。【结果】与正常组比较,抑郁模型组大鼠海马CA3、DG区出现典型的凋亡细胞,且凋亡率及凋亡细胞数量差异均有统计学意义(P<0.01)。与模型组比较,β-细辛醚组与氟西汀组海马区凋亡细胞数量及凋亡率均显著降低(P<0.01)。【结论】β-细辛醚可能通过减少细胞凋亡的方式对抑郁症大鼠海马区细胞起到保护作用。
【Objective】 To investigate the effect of β-asarone on hippocampus cells of depression rats. 【Methods】 Eighty SD mature male rats were randomly divided into 4 groups: normal group, model group, fluoxetine group (1.2 mg / kg), β-asarone group (25 mg / kg ). Except for the normal group, the other three groups were given the model of depressive depressive rats separately. After 21 days, the number and morphology of apoptotic cells in each group were detected by flow cytometry and TUNEL. 【Results】 Compared with normal group, typical apoptotic cells appeared in CA3 and DG of hippocampus of depression model group, and the difference between apoptosis rate and apoptotic cells was statistically significant (P <0.01). Compared with the model group, the number of apoptotic cells and the apoptosis rate in hippocampus of β-asarone group and fluoxetine group were significantly decreased (P <0.01). 【Conclusion】 β-asarone may protect the hippocampus cells of depression rats by reducing the apoptosis.