In this study,the effects of bio-organic fertilizer with antagonistic bacteria against tobacco bacterial wilt on soil microbial communities and disease resistance of tobacco were investigated by field experiment. The results showed that the incidence of tobacco bacterial wilt in bio-organic fertilizer treatments( T3 and T4)decreased remarkably among four treatments in the field. Compared with the local conventional fertilization group,the incidence of tobacco bacterial wilt was reduced by 21. 9% and 25. 0% in T3 and T4,respectively; the yield of flue-cured tobacco was improved by 5. 7% and 5. 3%,respectively; the proportion of midhigh grade tobacco leaves increased by 2. 3% and 2. 6%,respectively. After application of bio-organic fertilizer with antagonistic bacteria against tobacco bacterial wilt,rhizosphere soil microbial communities exhibited vast amount and abundant species; the amount of rhizosphere soil bacteria of infected tobacco plants was improved by 218. 5% with fewer species. It could be concluded that the application of bio-organic fertilizer with antagonistic bacteria against tobacco bacterial wilt could improve the ecological environment of tobacco field,inhibit the growth of pathogenic bacteria,decrease the incidence of tobacco bacterial wilt,and enhance the quality of flue-cured tobacco. This study laid the foundation for further ecological prevention and control of soil-borne diseases of tobacco. In this study, the effects of bio-organic fertilizer with antagonistic bacteria against tobacco bacterial wilt on soil microbial communities and disease resistance of tobacco were investigated by field experiment. The results showed that the incidence of tobacco bacterial wilt in bio-organic fertilizer treatments ( Compared with the local conventional fertilization group, the incidence of tobacco bacterial wilt was reduced by 21.9% and 25. 0% in T3 and T4, respectively; the yield of flue -cured tobacco was improved by 5. 7% and 5.3% respectively; the proportion of midhigh grade tobacco leaves increased by 2.3% and 2.6% respectively. After application of bio-organic fertilizer with antagonistic bacteria against tobacco bacterial wilt, rhizosphere soil microbial communities exhibition vast amount and abundant species; the amount of rhizosphere soil bacteria of infected tobacco plants was improved by 218. 5% with fewer specie s. It could be concluded that the application of bio-organic fertilizer with antagonistic bacteria against tobacco bacterial wilt could improve the ecological environment of tobacco field, inhibit the growth of pathogenic bacteria, decrease the incidence of tobacco bacterial wilt, and enhance the quality of flue-cured tobacco. This study laid the foundation for further ecological prevention and control of soil-borne diseases of tobacco.