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目的:研究后肢去负荷干预后不同时间的跑台训练对大鼠股骨骨密度和骨代谢指标的影响。方法:9周龄Wistar雌性大鼠64只随机分为8组,每组8只。Group 1:尾部悬吊4周,处死;Group 2和Group3:尾部悬吊4周,之后分别跑台训练4周和8周,处死;Group 4和Group 5:尾部悬吊4周,之后分别自由活动4周和8周,处死;Group 6、Group 7和Group 8:正常饲养,不进行尾部悬吊,分别于分组4周后、8周后和12周后处死。测试各组大鼠股骨骨密度和血清骨代谢指标。结果:与其对应周龄的阴性对照组相比,尾部悬吊组大鼠血钙和抗酒石酸酸性磷酸酶5b显著升高,血清碱性磷酸酶和骨钙素无显著差异,骨密度显著降低;恢复正常活动后,大鼠血清碱性磷酸酶和骨钙素显著升高,血钙和抗酒石酸酸性磷酸酶5b显著下降,骨密度显著升高;与自由活动组相比,跑台训练组大鼠上述骨代谢指标和骨密度的变化更显著。结论:后肢去负荷导致大鼠骨吸收作用显著增强,使骨密度下降;后肢恢复正常活动后,大鼠的骨形成作用显著增强,并且骨吸收作用也逐渐降至正常水平,骨密度逐渐升高;跑台训练可加速大鼠后肢去负荷干预后骨代谢的改变和骨密度的恢复。
OBJECTIVE: To study the effects of treadmill training on the femoral bone mineral density (BMD) Methods: Sixty-four Wistar female rats aged 9 weeks were randomly divided into 8 groups with 8 rats in each group. Group 1: tail suspension for 4 weeks and sacrifice; Group 2 and Group 3: tail suspension for 4 weeks, followed by treadmill training for 4 weeks and 8 weeks, respectively; Group 4 and Group 5: tail suspension for 4 weeks, Animals were sacrificed at 4 weeks and 8 weeks respectively. Group 6, Group 7 and Group 8 were maintained in normal conditions without tail suspension and were sacrificed 4 weeks, 8 weeks and 12 weeks later. The femur BMD and serum bone metabolism index of the rats in each group were tested. Results: Compared with the negative control group, the serum calcium and tartrate - resistant acid phosphatase 5b in the tail suspension group were significantly increased, but there was no significant difference in serum alkaline phosphatase and osteocalcin, but the bone mineral density was significantly decreased. Serum alkaline phosphatase and osteocalcin significantly increased after resumption of normal activity, while serum calcium and tartrate-resistant acid phosphatase 5b significantly decreased and bone mineral density increased significantly. Compared with free activity group, treadmill training group The above indexes of bone metabolism and bone mineral density changed more significantly. CONCLUSION: The bone resorption of hind limbs was significantly increased and the BMD of the rats decreased significantly. The bone formation was significantly enhanced and the bone resorption gradually decreased to normal level and the BMD gradually increased after the hind limbs returned to normal activity Treadmill training can accelerate the change of bone metabolism and the recovery of bone mineral density after hindlimb unloading in rats.