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目的测定不同种类鹿茸尖部和基部水溶性成分含量,并分析其含量差异。方法对传统方法加工的鹿茸进行水溶性成分提取,采取凝胶过滤层析方法,以细胞色素C为内参,采用Superdex~(TM)75 10/300 GL凝胶过滤层析柱,缓冲液使用0.05mol·L~(-1)PBS+0.3 mol·L~(-1)NaCl,流速0.5 ml/min,检测波长280 nm,通过峰下面积积分,计算水溶性成分含量。结果实验建立了水溶性成分含量的计算方法。经计算水溶性成分含量分别为梅花鹿二杠茸尖部30.50%,基部为6.77%;梅花鹿三杈茸尖部29.69%,基部为5.98%;马鹿四杈茸尖部28.27%,基部为3.22%。结论水溶性成分含量在鹿茸尖部和基部差异明显,梅花鹿二杠茸、梅花鹿三杈茸和马鹿四杈茸的尖部分别为基部的4.5倍,5倍和9倍;从不同种类鹿茸的水溶性成分含量上看,梅花鹿二杠茸优于梅花鹿三杈茸优于马鹿四杈茸,与传统分类相一致。
Objective To determine the content of water-soluble components at the tip and base of different species of pilose antler, and to analyze the content differences. Methods The water-soluble constituents of the pilose antler processed by the traditional method were extracted by gel filtration chromatography with Cytochrome C as internal reference and Superdex ~ (TM) 75 10/300 GL gel filtration chromatography column. The buffer was treated with 0.05 mol·L -1 PBS + 0.3 mol·L -1 NaCl at a flow rate of 0.5 ml / min. The detection wavelength was 280 nm. The content of water-soluble components was calculated by area under the peak. Results The experiment established the method of calculating the content of water-soluble components. The content of water-soluble components was 30.50% at the top of the sika deer, 6.77% at the base, 29.69% at the top of the three-sika deer, 5.98% at the base, 28.27% at the top of the deer with four twigs, and 3.22% at the base . Conclusion The contents of water-soluble components in pilose antler were significantly different at the tip and the base. The tips of the two parts of the two kinds of velvet antler, the three-prong deer and the red deer were 4.5 times, 5 times and 9 times the base, respectively. The content of water-soluble ingredients point of view, sika deer two barrels Velvet superior to sika deer three bracts better than red deer four twigs, consistent with the traditional classification.