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背景:在成年大鼠的脑室下层存在神经元前体细胞,这些细胞持续不断沿着嘴侧迁移流不停地向嗅球迁移并分化嗅球内的中间神经元,阻断嘴侧迁移流对这些细胞的迁移以及分化是否产生影响尚不得而知。目的:探讨嘴侧迁移流阻断对脑室下层细胞迁移的影响。设计:随机对照实验。单位:中国科学技术大学生命科学学院;徐州医学院人体解剖学教研室。材料:实验于1997-09/1999-01在徐州医学院动物实验中心完成,选用成年雄性SD大鼠25只。随机分为嗅茎切断30d组(n=10)和60d组(n=15,其中5只作免疫组织化学)。方法:在嗅球后切断嗅茎,手术30,60d后将动物灌注固定处死,制作脑的连续石蜡切片,计量嗅球后份平面嘴侧迁移流的细胞密度和横截面积、用免疫组织化学的方法对嘴侧迁移流内的细胞进行定性分析。主要观察指标:嗅茎后份平面的细胞密度,横截面积和唾液酸-神经细胞黏附分子的表达。结果:纳入25只SD大鼠,因手术动物死亡和嗅茎切断手术失败,用于分析的动物只有13只,30d组5只,60d组8只(其中用于免疫组织化学的3只)。①嗅茎切断后嗅茎后份平面嘴侧迁移流形态学及数量变化:在Nissl染色的切片上,嘴侧迁移流细胞较密集,染色深,和周围组织区别明显。未见明显的核固缩、核碎片等细胞死亡的特征。嗅茎切断30d组和60d组手术侧嗅茎后份平面的细胞密度均高于对照侧(P<0.01),60d组手术侧嘴侧迁移流在嗅茎后份平面的相对细胞密度高于30d组手术侧的细胞密度(P<0.01)。两组手术侧横截面积均大于对照侧(P<0.01),但嗅茎切断60d组和30d组手术侧无差异(P>0.05)。②嗅茎切断手术后嘴侧迁移流细胞的免疫组织化学反应:嗅茎切断60d后,嗅茎后份平面嘴侧迁移流内积聚的细胞表现为多唾液酸-神经细胞黏附分子阳性。结论:切断嗅茎导致嘴侧迁移流内神经元前体细胞在切断平面的尾侧积聚,这些集聚的细胞可能发生分化。
BACKGROUND: Neuronal progenitor cells exist in the subventricular space of adult rats. These cells continue to migrate along the mouth side to the olfactory bulb and differentiate into interneurons in the olfactory bulb, blocking the flow of migratory cells to these cells It is unclear whether the migration and differentiation have an impact. Objective: To investigate the effect of occlusion of migratory mouth on the migration of subventricular layer cells. Design: Randomized controlled experiment. Unit: College of Life Sciences, University of Science and Technology of China; Department of Human Anatomy, Xuzhou Medical College. MATERIALS: Experiments were performed at the Animal Experimental Center of Xuzhou Medical College from September 1997 to January 1999 with 25 adult male SD rats. The rats were randomly divided into three groups: olfactory stalk sever 30d (n = 10) and 60d (n = 15, of which 5 were immunohistochemistry). Methods: The olfactory bulb was cut off after the olfactory bulb. The animals were perfused and fixed at 30 and 60 days after surgery. Serial paraffin sections of the brain were made and the cell density and cross-sectional area of the migratory mouth of the posterior face of the olfactory bulb were measured. By immunohistochemistry Qualitative analysis of the cells within the mouth-side migration stream. MAIN OUTCOME MEASURES: Cell density, cross-sectional area and expression of sialic acid-nerve cell adhesion molecules at the posterior plane of the olfactory bulb. RESULTS: Twenty-five Sprague-Dawley rats were enrolled in this study. Totally 13 animals were sacrificed due to the death of the surgical animal and olfactory bulb occlusion. Only 5 animals were used in the 30-day group and 8 animals in the 60-day group, of which 3 were used for immunohistochemistry. ① Morphology and quantitative changes of migratory flow at the posterior face of the posterior face of the olfactory bulb after the olfactory bulb was cut off: On the Nissl-stained sections, the migratory cells on the mouth side are densely packed and deeply stained, distinct from the surrounding tissues. No obvious nuclear pyknosis, nuclear debris and other cell death characteristics. The cell density of the olfactory stump severing 30d and 60d groups was higher than that of the control (P <0.01), and the relative cell density at the posterior olfactory plane of the 60d group was higher than that at 30d Group surgery side of the cell density (P <0.01). The cross-sectional area of the two sides were larger than the control side (P <0.01), but there was no significant difference between the two groups (P> 0.05). (2) The immunohistochemical reaction of migratory cells on the mouth side after olfactory stem cell transection: 60 days after the olfactory stalk was sequestered, the cells accumulated in the migratory mouth at the posterior face of the olfactory bulb showed positive expression of polysialic acid-nerve cell adhesion molecules. Conclusion: Closing the olfactory stem leads to accumulation of neuronal precursor cells in the mouth-side migration stream on the caudal side of the cut-off plane, and these aggregated cells may differentiate.