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目的:用DetectCell染色人结肠癌细胞(HT-29)和小鼠胚胎成纤维细胞(C3H)细胞,探索DetectCell染色在细胞良恶性鉴别中的价值。方法:DetectCell染色试剂主要由含有酸性红,碱性绿和具有特殊反应亲和力的无花果属植物提取液组成。通过检测各主要染色试剂pH值、稀释浓度波长的吸光度比值(OD540/OD630)以控制染色试剂的检测标准,然后进行DetectCell染色观察细胞形态与颜色特征。结果:DetectCell主要染液的pH值和540 nm/630 nm波长处OD值均符合检测标准;DetectCell染色HT-29细胞和C3H细胞能保持原有细胞的形态,同时分别使纤维母细胞C3H被染成绿色,使癌细胞HT-29被染成红色。结论:DetectCell染色使癌细胞呈红色,非癌细胞呈绿色,并不影响细胞形态特征,因此该染色提供了颜色和形态双重分析功能。
OBJECTIVE: To detect the value of DetectCell staining in differentiating benign and malignant cells by using DetectCell staining of human colon cancer cells (HT-29) and mouse embryonic fibroblasts (C3H). Method: The DetectCell Staining Reagent consists of a fig extract containing acidic red, basic green and a special reaction affinity. By detecting the pH value of the major staining reagents, the absorbance ratio (OD540 / OD630) of the diluted concentration wavelength was used to control the staining reagent detection standard, and then the cell morphology and color characteristics were observed by DetectCell staining. Results: The pH values of DetectCell dye solution and OD value at 540 nm / 630 nm were both in accordance with the detection standards. DetectCell staining of HT-29 cells and C3H cells maintained the original cell morphology, It was dyed green so that the cancer cell HT-29 was dyed red. CONCLUSIONS: DetectCell staining causes cancer cells to be red and non-cancer cells to be green without affecting the morphological characteristics of the cells, thus providing a dual color and morphological analysis.