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目的:探讨TMPRSS4基因对结肠癌细胞增殖及迁移的影响。方法 :采用免疫组化方法检测结肠直肠癌组织和正常肠上皮中TMPRSS4的表达,并应用RT-PCR和Western印迹法检测结肠癌细胞株中TMPRSS4的表达,通过RNAi方法下调结肠癌细胞SW480中TMPRSS4的表达后,进一步采用CCK-8法、细胞划痕和transwell迁移试验检测细胞增殖、运动及迁移能力的改变。通过流式细胞仪检测TMPRSS4下调后对细胞凋亡的影响。结果:结肠直肠癌高表达TMPRSS4;肠癌细胞株SW480在mRNA及蛋白水平均高表达TMPRSS4。下调TMPRSS4能抑制SW480的增殖能力(P<0.05),并诱导细胞凋亡增加(P<0.05)。此外,下调TMPRSS4降低SW480细胞的迁移能力(P<0.05)。结论:在高表达细胞株中下调TMPRSS4有助于抑制肠癌细胞增殖,增加细胞凋亡并降低细胞的迁移能力。
Objective: To investigate the effect of TMPRSS4 gene on the proliferation and migration of colon cancer cells. Methods: The expression of TMPRSS4 in colorectal cancer tissues and normal intestinal epithelium was detected by immunohistochemistry. The expression of TMPRSS4 in colon cancer cell lines was detected by RT-PCR and Western blotting. The expression of TMPRSS4 in colon cancer cells SW480 was down-regulated by RNAi CCK-8 method, cell scratch and transwell migration assay were further used to detect cell proliferation, motility and migration abilities. The effect of TMPRSS4 on apoptosis was detected by flow cytometry. Results: TMPRSS4 was overexpressed in colorectal cancer and TMPRSS4 was expressed in SW480 at mRNA and protein levels. Down-regulation of TMPRSS4 could inhibit the proliferation of SW480 (P <0.05) and induce apoptosis (P <0.05). In addition, down-regulation of TMPRSS4 reduced SW480 cell migration (P <0.05). Conclusion: Down-regulation of TMPRSS4 in high-expression cell lines can inhibit the proliferation of colorectal cancer cells, increase apoptosis and decrease cell migration.