论文部分内容阅读
建立了超高效液相色谱-二极管阵列检测器(UPLC-PDA)同时分离测定香精香料中糖精钠,芝麻酚,酸性橙Ⅱ,碱性嫩黄O的方法。样品经过10%的甲醇水溶液(含1%氨水)提取后,以Waters BEHC18(1.7μm,2.1mm×50mm)为色谱柱,5mmol/L乙酸铵(含0.1%乙酸)和乙腈(ACN)为流动相,200—500nm波长范围内进行扫描检测。结果表明,在5min以内4种目标物质得到了较好的分离,在0.5—20mg/L范围内具有较好的线性(r>0.9950),检出限为0.08—0.61mg/L,3种浓度水平的加标回收率保持在85.3%—110.3%之间,相对标准偏差在6%以内。该方法操作简单,灵敏度高,可以满足香精香料中4种物质的同时检测。
A method for simultaneous determination of sodium saccharin, sesamol, acid orange Ⅱ and basic yellow O in flavor and fragrance by UPLC-PDA was established. After the sample was extracted with 10% aqueous methanol solution (containing 1% ammonia), the sample was eluted with a mobile phase of Waters BEHC18 (1.7μm, 2.1mm × 50mm), 5mmol / L ammonium acetate with 0.1% acetic acid and ACN Phase, 200-500nm wavelength range for scanning detection. The results showed that 4 kinds of target substances were well separated within 5 minutes, with good linearity (r> 0.9950) in the range of 0.5-20 mg / L and detection limits of 0.08-0.61 mg / L. The three concentrations The recoveries of standard spikes were between 85.3% and 110.3% with relative standard deviations within 6%. The method is simple in operation and high in sensitivity, and can satisfy the simultaneous detection of four substances in flavor and fragrance.