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目的采用多柔比星(doxorubicin,DOX)制备心肌损伤动物模型,评价各种检测心功能变化方法的意义。方法 14只新西兰大白兔,DOX耳缘静脉注射,每周3mg/kg,共10周。分别于给药前、第4周末及实验结束时测定血清肌钙蛋白Ⅰ(cTnI)和脑钠肽(BNP)水平,彩色多普勒超声心动图检测心功能变化,并观察心肌组织病理形态学改变及心肌细胞凋亡情况。结果使用DOX前后对比,血清cTnI和BNP浓度升高(P<0.05);左室射血分数(LVEF)和左室短轴缩短率(LVFS)下降(P<0.05);心肌组织病理显示心肌细胞出现不同程度的空泡变性与水肿,细胞间隙明显增宽,大量炎性细胞浸润。心肌细胞凋亡明显增加。结论结合心脏超声检查和血清cTnI、BNP指标检测可判断心肌损伤程度。
OBJECTIVE: To establish a rat model of myocardial injury by doxorubicin (DOX) and evaluate the significance of various methods for detecting cardiac function changes. Methods Fourteen New Zealand white rabbits, DOX, were injected intravenously 3 mg / kg weekly for 10 weeks. Serum levels of troponin Ⅰ (cTnI) and brain natriuretic peptide (BNP) were measured before administration, at the end of the 4th week, and at the end of the experiment. Changes of cardiac function were detected by color Doppler echocardiography, and myocardial histopathology Change and cardiomyocyte apoptosis. Results Compared with before and after DOX, the concentrations of cTnI and BNP in serum increased (P <0.05), the left ventricular ejection fraction (LVEF) and left ventricular fractional shortening (LVFS) decreased (P <0.05) There are different degrees of vacuolar degeneration and edema, cell gap was significantly widened, a large number of inflammatory cell infiltration. Myocardial apoptosis increased significantly. Conclusion Combined with echocardiography and serum cTnI, BNP index can determine the degree of myocardial injury.