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以99个百合远缘杂种F1代-麝香百合×亚洲百合(LA)杂种为试材,采用栽培基质接种病原菌的方法对杂种后代的镰刀菌抗性进行了鉴定。结果表明:百合无性系温室人工接种技术成功应用于该远缘杂种,其母本麝香百合‘White Fox’对镰刀菌表现出高感,而父本亚洲百合‘Connecticut King’具有较强抗性,并成功遗传给杂种后代,在后代基因型中实现一系列分离;在后代杂种群体中,大部分基因型对镰刀菌表现出不同程度的抗性,F1代镰刀菌抗性基因表达率为70.1%。表明镰刀菌抗性由数量基因控制,通过杂种系间杂交实现百合镰刀菌抗性的渐渗育种切实可行。
Fusarium resistance of hybrid offspring was identified by inoculation of pathogenic bacteria with 99 lilies of F1 hybrid - Musk lily × Lily (LA). The results showed that artificial inoculation technique of lily clonal greenhouse was successfully applied to the distant hybrids. The female white lily ’White Fox’ showed high sensitivity to Fusarium, while the male lily ’Connecticut King’ had strong resistance, And successfully inherited to the hybrid offspring, to achieve a series of segregation in the offspring genotype. In the offspring hybrid population, most of the genotypes showed different degrees of resistance to Fusarium, the Fusarium resistance gene expression rate of F1 was 70.1% . Indicating that Fusarium resistance is controlled by quantitative genes, it is practicable to carry out introgressing breeding of Fusarium lactis resistance by crossing between hybrids.