论文部分内容阅读
目的 通过检测急性心肌梗死 (acutemyocardialinfarceion ,AMI)患者血清环氧化酶 - 2 (COX- 2 )活性 ,探讨COX - 2与AMI病情的关系。方法 将样本分为AMI组和正常对照组 ,AMI组均于急性发病后 1 (治疗前 )、 2、 3、 7、 1 4d各抽一次空腹肘静脉血 2 0ml:正常对照组从体检健康人群中抽空腹肘静脉血 2 0ml,酶联免疫吸附双抗体夹心法检测血清COX - 2的活性。结果 COX - 2活性在AMI死亡组明显高于存活组 (P <0 0 5 ) ;AMI组COX - 2活性在 1、2、 3d明显高于正常对照组 (P均小于 0 0 1 ) ,于 7、 1 4d它们与对照组比较则均差异显著性 (P >0 0 5 ) ;且COX - 2的活性变化与AMI患者的血清CK -MB含量的变化、临床积分、梗死面积均呈明显的正相关性。结论 COX - 2活性与AMI患者的病情变化相一致 ,随病情的严重程度增加而升高 ,病情减轻而下降 ,提示COX - 2可能参与了AMI的病理生理过程 ,COX - 2可能可作为AMI患者病情监测和预后的参考指标之一
Objective To explore the relationship between COX - 2 and AMI by detecting serum cyclooxygenase - 2 (COX - 2) activity in patients with acute myocardial infarction (AMI). Methods The samples were divided into two groups: AMI group and normal control group. AMI group was given 1 ml of fasting elbow venous blood at 1, 2, 3, 7 and 14 days after acute onset (normal control group) 20 mL of fasting venous blood was drawn and serum COX - 2 activity was measured by enzyme - linked immunosorbent assay (ELISA). Results The COX - 2 activity in AMI group was significantly higher than that in survivor group (P <0.05). The COX - 2 activity in AMI group was significantly higher than that in control group at 1, 2 and 3 days (P <0.01) (P> 0.05). The changes of COX - 2 activity and serum CK-MB in AMI patients showed significant differences in clinical scores and infarct size Positive correlation. Conclusions The COX - 2 activity is consistent with the changes of the disease in AMI patients, with the severity of the disease increasing and the severity of the disease decreasing, suggesting COX - 2 may be involved in the pathophysiological process of AMI. COX - 2 may be used as AMI Disease monitoring and prognosis of the reference index