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The expression level of suppressor of cytokine signaling-3(SOCS-3) in human renal carcinoma and its methylation state were investigated. Reverse transcription-polymerase chain reaction(RT-PCR), immuocytochemistry, immunohistochemistry and Western blot were used to detect the expression level of SOCS-3, and the methylation of SOCS gene was investigated by methylation specific PCR in the tissues of 15 cases of renal carcinoma. Compared to those of the normal renal cell line and specimens , the expression level of SOCS-3 in renal carcinoma was significantly lower or can’t be detected(P<0.01). And the methylation of SOCS-3 gene in the tissue of renal carcinoma was significantly higher. The expression of SOCS-3 gene is significantly lower in renal carcinoma and the high methylation of the promoter island of SOCS-3 gene is associated with the lower expression of SOCS-3 gene. It may be one of main mechanisms for the development and progress of renal carcinoma.
The expression level of suppressor of cytokine signaling-3 (SOCS-3) in human renal carcinoma and its methylation state were investigated. Reverse transcription-polymerase chain reaction (RT-PCR), immuocytochemistry, immunohistochemistry and Western blot were used to detect the expression level of SOCS-3, and the methylation of SOCS gene was investigated by methylation specific PCR in the tissues of 15 cases of renal carcinoma. Compared to those of the normal renal cell line and specimens, the expression level of SOCS-3 in renal carcinoma The expression of SOCS-3 gene is significantly lower in renal carcinoma and the high methylation (P <0.01). And the methylation of SOCS-3 gene in the tissue of renal carcinoma was significantly higher. of the promoter island of SOCS-3 gene is associated with the lower expression of SOCS-3 gene. It may be one of the main mechanisms for the development and progress of renal carcinoma.