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我们建立了基于多克隆抗促红细胞生成素抗体的 ELISA测定血清促红细胞生成素(Epo)方法,检测了各类病毒性肝炎患者的血清 Epo 含量。材料与方法一、研究对象正常对照组(NOR)40例为本院健康职工和实习进修人员;缺铁性贫血(IDA)对照组40例均无肝、肾疾病;急性肝炎(AH)45例,慢性活动型肝炎(CAP)42例,慢性迁延型肝炎(CPH)45例,重症肝炎(SH)17例和肝炎后肝硬化(CIR)18例,HCC 38例,以上病例由本院及广东医学院附院提供。肝炎诊断符合1990年病毒性肝炎学术会议修订的诊断分型标准,HCC 患者均经双份肝活检标本光镜和电镜检查支持,肿瘤直径大小取 B 超、CT、肝动脉造影、核磁共振4项中的2～4项测量的最大值。二、标本所有对象均取静脉血,血液常规项目由自动血液分析仪检测,Epo 项目则分离血清,-70℃保存待检。三、Elisa 测定血清 Epo 方法方法由本所建立(另文发表),包被抗体是我们经亲和纯化的多克隆抗 Epo,浓度2ug/ml,100ul/孔;洗板,加重组
We have established a method based on polyclonal anti-erythropoietin antibody for the determination of serum erythropoietin (Epo) in patients with various types of viral hepatitis Epo content. MATERIALS AND METHODS A. Study object Forty cases of normal control group (NOR) were hospital-based health workers and internship fellows; 40 cases of IDA-free control group had no liver or kidney disease; 45 cases of acute hepatitis (AH) , 42 cases of chronic active hepatitis (CAP), 45 cases of chronic protracted hepatitis (CPH), 17 cases of severe hepatitis (SH), 18 cases of cirrhosis of cirrhosis (CIR) and 38 cases of HCC. Hospital Affiliated Hospital provided. The diagnosis of hepatitis was in line with the diagnostic classification standard revised by the Viral Hepatitis Symposium in 1990. HCC patients were supported by light and electron microscopic examination of double liver biopsy specimens. The diameters of the tumors were B ultrasound, CT, hepatic artery angiography and four nuclear magnetic resonance 2 to 4 in the measurement of the maximum. Second, all specimens were taken venous blood, routine blood test by automatic blood analyzer, Epo project is the separation of serum, -70 ℃ for inspection. Third, Elisa method for the determination of serum Epo by the establishment of (published separately), coating antibody is our affinity purified polyclonal anti-Epo, concentration 2ug / ml, 100ul / well;