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目的:从化学成分和化学性脑损伤保护方面评价当归挥发油与川芎挥发油的异同。方法:采用水蒸气蒸馏法分别提取当归和川芎挥发油,运用GC-MS法测定二者挥发油的成分及含量;将70只昆明种小鼠随机分为7组,分别为空白组、、吡拉西坦片200 mg/kg剂量组、当归挥发油40 mg/kg剂量组、当归挥发油20 mg/kg剂量组、川芎挥发油40 mg/kg剂量组、川芎挥发油20 mg/kg剂量组,连续灌胃给药7 d,于末次给药2 h后,腹腔注射NaNO_2(800 mg/kg),定磷法测定Na~+-K~+-ATPase,Ca~(2+)-Mg~(2+)-ATPase,乙酰胆碱酯酶(AchE)水平。结果:从当归和川芎挥发油中各鉴定出14和16个化学成分,其中共有成分6个,两者的主要成分均为Z-藁苯内酯,且含量均超过50%。当归油和川芎挥发油40 mg/kg剂量组均可升高Na~+-K~+-ATPase,Ca~(2+)-Mg~(2+)-ATPase水平,降低乙酰胆碱酯酶水平。结论:二者化学成分组成存在差异,但主成分种类及含量相似,且二者挥发油在化学性脑缺氧模型下,均可通过改善小鼠能量代谢和中枢胆碱能神经系统的功能,保护缺氧组织和维持小鼠正常的能量活动。
OBJECTIVE: To evaluate the similarities and differences between the volatile oil of angelica sinensis and volatile oil of Ligusticum chuanxiong Hort from chemical composition and chemical brain injury protection. Methods: The essential oil of Radix Angelica Sinensis and Ligusticum Chuanxiong Hort. Was extracted by steam distillation. The composition and content of the volatile oil in both extracts were determined by GC-MS. Seventy Kunming mice were randomly divided into 7 groups, In the group of 200 mg / kg, Angelica sinensis 40 mg / kg, Angelica sinensis volatile oil 20 mg / kg, Chuanxiong volatile oil 40 mg / kg and Chuanxiong volatile oil 20 mg / kg, At 7 days after the last administration, NaNO_2 (800 mg / kg) was injected intraperitoneally. The contents of Na ~ + -K ~ + -ATPase, Ca ~ (2 +) - Mg ~ (2 +) - ATPase , Acetylcholinesterase (AchE) levels. Results: 14 and 16 chemical constituents were identified from the volatile oil of Angelica sinensis and Rhizoma liguttii. There were 6 common constituents in this study. The main constituents of both were Z-藁 phenanthrolides, and their contents were over 50%. Angelica oil and Ligusticum chuanxiong volatile oil 40 mg / kg dose group can increase the level of Na ~ + -K ~ + -ATPase, Ca ~ (2 +) - Mg ~ (2 +) - ATPase and reduce the level of acetylcholinesterase. Conclusion: There are differences in the chemical composition between the two components, but their main components are similar in type and content. Both of them can protect the energy metabolism and the central cholinergic nervous system of mice in chemical brain hypoxia model Hypoxic tissue and maintain normal energy activity in mice.